Staurosporine

Name: Staurosporine
Brand: Tocris Bioscience
SKU: 1285
Price: 176 USD

Tocris Bioscience a Bio-Techne Brand

Catalog # 1285

Citations (20)

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Key Product Details

Description:	Non-selective protein kinase inhibitor
Alternative Names:	AM-2282
Chemical Name:	[9S-(9α,10β,11β,13α)]-2,3,10,11,12,13-Hexahydro-10-methoxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo[1,2,3-gh:3',2',1'-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one
Purity:	≥98% (HPLC)
Molecular Weight:	466.54

View Full Technical Details for Staurosporine

Biological Activity for Staurosporine

Staurosporine is a broad spectrum protein kinase inhibitor. Enzymes inhibited include protein kinase C, p^60v-src tyrosine protein kinase, protein kinase A, and CaM kinase II (IC₅₀ values are 3nM, 6 nM, 7 nM and 20 nM, respectively). Staurosporine reduces nuclear myosin heavy chain 9 phosphorylation which inhibits gastric cancer cell progression in transgenic mouse models. Staurosporine inhibits cell viability and promotes apoptosis in oral and pancreatic cancer cells. Staurosporine also enhances efficiency of lentiviral transduction of human hematopoietic stem and progenitor cells by 2-fold, induces dopaminergic axonal outgrowth in vitro and triggers mitophagy. Staurosporine has high affinity (K_d = 100 nM) for the yjdF aptamer. The riboswitch function of yjdF motif RNAs is activated by Staurosporine and leads to robust reporter gene expressions in B. subtilis.

Staurosporine synthesized to Ancillary Material Grade is also available.

Technical Data for Staurosporine

M. Wt	466.54
Formula	C₂₈H₂₆N₄O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	62996-74-1
PubChem ID	44259
InChi Key	HKSZLNNOFSGOKW-FYTWVXJKSA-N
Smiles	O=C(NC6)C1=C6C4=C3C2=C1C8=C(C=CC=C8)N2[C@](C[C@@H](NC)[C@H]7OC)([H])O[C@]7(C)N3C5=CC=CC=C45

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Staurosporine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	23.33	50

Preparing Stock Solutions for Staurosporine

The following data is based on the product molecular weight 466.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.29 mL	21.43 mL	42.87 mL
2.5 mM	0.86 mL	4.29 mL	8.57 mL
5 mM	0.43 mL	2.14 mL	4.29 mL
25 mM	0.09 mL	0.43 mL	0.86 mL

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References for Staurosporine

References are publications that support the biological activity of the product.

Ye Nuclear MYH9-induced CTNNB1 transcription, targeted by staurosporin, promotes gastric cancer cell anoikis resistance and metastasis. Theranostics 2020 PMID: 32685004
Malsy Staurosporine induces apoptosis in pancreatic carcinoma cells PaTu 8988t and Panc-1 via the intrinsic signaling pathway. Eur.J.Med.Res. 2019 PMID: 30686270
Lewis Staurosporine increases lentiviral vector transduction efficiency of human hematopoietic stem and progenitor cells. Mol.Ther.Methods Clin.Dev. 2018 PMID: 30038935
Li The yjdF riboswitch candidate regulates gene expression by binding diverse azaaromatic compounds. RNA 2016 PMID: 26843526
Wakita Staurosporine induces dopaminergic neurite outgrowth through AMP-activated protein kinase/mammalian target of rapamycin signaling pathway. Neuropharmacology 2014 PMID: 24067927
Zhang Effect of protein kinase C alpha, caspase-3, and survivin on apoptosis of oral cancer cells induced by staurosporine. Acta Pharmacol.Sinica 2005 PMID: 16225760
Yanagihara Staurosporine: an effective inhibitor for Ca²⁺/calmodulin-dependent protein kinase II. J.Neurochem. 1991 PMID: 1846174
Ruegg and Burgess Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases. TiPS 1989 PMID: 2672462
Tamaoki Staurosporine, a potent inhibitor of phospholipid/Ca²⁺ dependent protein kinase. Biochem.Biophys.Res.Commun. 1986 PMID: 3457562

Citations for Staurosporine (20)

Showing 1 - 10 of 20 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Staurosporine include:

Xu et al. (2017) Matrine induces RIP3-dependent necroptosis in cholangiocarcinoma cells. Cell Death Discov. 3:16096
PMID: 28179994
Hejna et al. (2017) High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells. Sci Rep. 7:11943
PMID: 28931937
Mayer et al. (2017) The microanatomic segregation of selection by apoptosis in the germinal center. Science. 358
PMID: 28935768
Valvezan et al. (2017) mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell. 32:624
PMID: 29056426
Chen et al. (2016) Androgen-Sensitized Apoptosis of HPr-1AR Human Prostate Epithelial Cells. Mol Pharmacol. 11:e0156145
PMID: 27203692
Park et al. (2016) Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol. 17:914
PMID: 27270401
Hayes et al. (2016) Integrative genomic and functional analysis of human oral squamous cell carcinoma cell lines reveals synergistic effects of FAT1 and CASP8 inactivation. Cancer Lett. 383:106
PMID: 27693639
Piccart et al. (2015) Neurotensin Induces Presynaptic Depression of D2 DA Autoreceptor-Mediated Neurotransmission in Midbrain DArgic Neurons. J Neurosci. 35:11144
PMID: 26245975
Sonamoto et al. (2015) Identification of a DYRK1A Inhibitor that Induces Degradation of the Target Kinase using Co-chaperone CDC37 fused with Luciferase nanoKAZ. Eneuro. 5:12728
PMID: 26234946
Faulkner et al. (2015) FMRP regulates neurogenesis in vivo in Xenopus laevis tadpoles. PLoS One. 2:e0055
PMID: 25844398
Dobson et al. (2015) Caffeine Modulates Vesicle Release and Recovery at Cerebellar Parallel Fibre Terminals, Independently of Calcium and Cyclic AMP Signalling. Eur J Neurosci. 10:e0125974
PMID: 25933382
O'Donnell et al. (2015) IF. gamma induces protective non-canonical signaling pathways in primary neurons. J Neurochem. 135:309
PMID: 26190522
Valentine et al. (2014) Small molecule screen yields inhibitors of Pseudomonas homoserine lactone-induced host responses. Cell Microbiol. 16:1
PMID: 23910799
Schönbrunn et al. (2013) Development of highly potent and selective diaminothiazole inhibitors of cyclin-dependent kinases. J Med Chem. 56:3768
PMID: 23600925
Barcomb et al. (2013) Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. J Biol Chem. 84:834
PMID: 24056996
Jackson et al. (2013) Pharmacological inhibition of pleckstrin homology domain leucine-rich repeat protein phosphatase is neuroprotective: differential effects on astrocytes. J Pharmacol Exp Ther. 347:516
PMID: 24023368
Kawamoto et al. (2012) Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults. PLoS One. 45:58
PMID: 22124704
Valero et al. (2011) Contractile effect of tachykinins on rabbit small intestine. Acta Pharmacol Sin. 32:487
PMID: 21441943
Kondo et al. (2010) Poly(ADP-ribose) polymerase (PARP)-1-independent apoptosis-inducing factor (AIF) release and cell death are induced by eleostearic acid and blocked by α-tocopherol and MEK inhibition. Braz J Med Biol Res. 285:13079
PMID: 20177052
Chen et al. (2006) N-myc down-regulated gene 1 modulates the response of term human trophoblasts to hypoxic injury. J Biol Chem. 281:2764
PMID: 16314423

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