SGC UBD253 New

Name: SGC UBD253
Brand: Tocris Bioscience
SKU: 8074

Tocris Bioscience, part of Bio-Techne | Catalog # 8074

COA / SDS

Potent and selective HDAC6-UBD antagonist; does not inhibit HDAC6 catalytic activity

Key Product Details

Chemical Name:	3-(8-Chloro-3-(2-((2-methoxybenzyl)amino)-2-oxoethyl)-4-oxo-3,4-dihydroquinazolin-2-yl)propanoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	429.86

View Full Technical Details for SGC UBD253

Technical Data
Citations

Biological Activity for SGC UBD253

SGC UBD253 is a potent and selective histone deacetylase 6 (HDAC6-UBD) antagonist (K_D = 80 nM, 84 nM and 95 nM determined by ITC, SPR and FP, respectively, for the UBD domain; K_D = 0.26 μM and 0.44 μM determined by SPR and FP, respectively, for full length HDAC6). Displays 50-fold selectivity for HDAC6 over other UBD proteins, with the exception of USP16 (15-fold). In live cells, it significantly decreases the interaction between HDAC6 and ISG15 (EC₅₀ = 1.9 μM in a NanoBRET assay). Treatment with SGC UBD253 does not inhibit the catalyitic activity of HDAC6.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the SGC UBD253 probe summary on the SGC website.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of SGC UBD253 is reviewed on the chemical probes website.

Technical Data for SGC UBD253

M. Wt	429.86
Formula	C₂₁H₂₀ClN₃O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	3033200-03-9
PubChem ID	164946806
InChi Key	YLHSFRZHEMHKAF-UHFFFAOYSA-N
Smiles	O=C1C2=CC=CC(Cl)=C2N=C(N1CC(NCC3=CC=CC=C3OC)=O)CCC(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SGC UBD253

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	0.43	1

Preparing Stock Solutions for SGC UBD253

The following data is based on the product molecular weight 429.86.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.01 mM	232.63 mL	1,163.17 mL	2,326.34 mL
0.05 mM	46.53 mL	232.63 mL	465.27 mL
0.1 mM	23.26 mL	116.32 mL	232.63 mL
0.5 mM	4.65 mL	23.26 mL	46.53 mL

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References for SGC UBD253

References are publications that support the biological activity of the product.

Harding Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6. J.Med.Chem. 2023 PMID: 37499118