RuBi-Glutamate

Name: RuBi-Glutamate
Brand: Tocris Bioscience
SKU: 3574
Rating: 5 (1 reviews)

Catalog # 3574 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (6)

COA / SDS

Caged glutamate; excited by visible wavelengths

Key Product Details

Description:	Caged glutamate; excited by visible wavelengths
Chemical Name:	(bis(2,2'-Bipyridine-N,N')trimethylphosphine)-(S)-1-aminopropane-1,3-dicarboxylic acid ruthenium(2⁺) complex sodium hexafluorophosphate salt
Molecular Weight:	970.54

View Full Technical Details for RuBi-Glutamate

Technical Data
Citations (6) / Reviews (1)

Biological Activity for RuBi-Glutamate

RuBi-Glutamate is a ruthenium-bipyridine-trimethylphosphine caged glutamate that can be excited by visible wavelengths and releases glutamate after one- or two-photon excitation. Has high quantum efficiency and can be used at low concentrations, partly avoiding blockade of GABAergic transmission seen with other caged glutamate compounds. Displays high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics.

MNI-caged-L-glutamate (Cat. No. 1490) also available.

Licensing Information

Sold under licence from Columbia University

Technical Data for RuBi-Glutamate

M. Wt	970.54
Formula	C₂₈H₃₂N₅O₄PRu.2NaPF₆
Storage	Store at -20°C
CAS Number	2417096-44-5
PubChem ID	90488860
InChi Key	SQQVSHWHIPVYSZ-UHFFFAOYSA-L
Smiles	[Na+].[Na+].[Ru++].CP(C)C.F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.NC(CCC([O-])=O)C([O-])=O.C1=CN=C(C=C1)C1=CC=CC=N1.C1=CN=C(C=C1)C1=CC=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RuBi-Glutamate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	19.41	20

Preparing Stock Solutions for RuBi-Glutamate

The following data is based on the product molecular weight 970.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	5.15 mL	25.76 mL	51.52 mL
1 mM	1.03 mL	5.15 mL	10.30 mL
2 mM	0.52 mL	2.58 mL	5.15 mL
10 mM	0.10 mL	0.52 mL	1.03 mL

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References for RuBi-Glutamate

References are publications that support the biological activity of the product.

Salierno A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes. J.Inorg.Biochem. 2010 PMID: 20060592
Fino RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines. Front.Neural Circuits 2009 PMID: 19506708

Citations for RuBi-Glutamate (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for RuBi-Glutamate include:

Tazerart et al. (2020) Spike-timing-dependent plasticity rule for single, clustered and distributed dendritic spines. Nat Commun. 11:4276
PMID: 32848151
Kazemipour et al. (2019) Kilohertz frame-rate two-photon tomography. Nat Methods. 16:778
PMID: 31363222
Mitchell et al. (2019) Probing Single Synapses via the Photolytic Release of Neurotransmitters Front Synaptic Neurosci. 11
PMID: 31354469
Paz et al. (2018) Inhibition of striatal cholinergic interneuron activity by the K_v7 opener retigabine and the nonsteroidal anti-inflammatory drug diclofenac. Neuropharmacology. 137:309
PMID: 29758221
Blaesse et al. (2015) μ-Opioid Receptor-Mediated Inhibition of Intercalated Neurons and Effect on Synaptic Transmission to the Central Amygdala. Neuroscience. 35:7317
PMID: 25972162
Roberto et al. (2013) Ruthenium polypyridyl phototriggers: from beginnings to perspectives. Philos Trans A Math Phys Eng Sci. 371:20120330
PMID: 23776299

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Customer Reviews for RuBi-Glutamate (1)

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Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Cell Line/Tissue: Periaqueductal gray

Recording from a DA neuron in the periaqueductal gray. A 6 x 6 grid was used to locally uncage Rubi-glutamate (300 micromolar) in an area surrounding the neuron. Representative trace showing the responses to each focal stimulation in current-clamp mode.

Light sensitive.

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