MNI-caged-L-glutamate

Product Description

MNI-caged-L-glutamate is a form of glutamate linked to a photo-protecting group, 4-methoxy-7-nitroindolinyl (MNI); it rapidly and efficiently releases L-glutamate (Cat. No. 0218) by photolysis (300 - 380 nm excitation) with a quantum yield in the 0.065-0.085 range. It is also suitable for use with two-photon uncaging microscopy (cross-section of 0.06 GM at 730 nm). MNI-caged-L-glutamate is optically compatible with other chromophores used for fluorescence imaging, such as GFP, YFP and most Ca²⁺ dyes. MNI-caged-L-glutamate is 2.5-fold more efficient at releasing L-glutamate than NI-caged L-glutamate. MNI-caged-L-glutamate is water-soluble, stable at neutral pH, highly resistant to hydrolysis and pharmacologically inactive at neuronal glutamate receptors and transporters (up to mM concentrations). MNI-caged-L-glutamate can be used for in situ studies of fast synaptic glutamate receptors.

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Licensing Information

Sold under license from the Medical Research Council

Solubility

Preparing Stock Solutions for MNI-caged-L-glutamate

The following data is based on the product molecular weight 323.30

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	6.19 mL	30.93 mL	61.86 mL
2.5 mM	1.24 mL	6.19 mL	12.37 mL
5 mM	0.62 mL	3.09 mL	6.19 mL
25 mM	0.12 mL	0.62 mL	1.24 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Ellis-Davies Two-Photon Uncaging of Glutamate Front Synaptic Neurosci. 2019 PMID: 30687075
• Palma-Cerda New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups. Neuropharmacology. 2012 PMID: 22609535
• Papageorgiou and Corrie Effects of aromatic substitutions on the photocleavage of 1-acyl-7-nitroindolines. Tetrahedron
• Matsuzaki Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons. Nat.Neurosci. 2001 PMID: 11687814
• Maier Comparative analysis of inhibitory effects of caged ligands for the NMDA receptor. J.Neurosci.Methods 2005 PMID: 15652611
• Canepari Photochemical and pharmacological evaluation of 7-nitroindolinyl- and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J.Neurosci.Methods 2001 PMID: 11640955