(RS)-3,5-DHPG application in brain slice preparation
Assay Type: Ex Vivo
(RS)-3,5-DHPG was bath applied and also pressure ejected at a concentration of up to 200 µM in a mouse brainstem slice preparation during whole-cell and perforated patch clamp. Results were consistent across different batch #s of (RS)-3,5-DHPG.
(RS)-3,5-DHPG is light and air sensitive and is not stable in alkaline solutions. It is best to use aliquots within a week. Aliquots should not have a brown coloration under proper storage conditions.