RG 108

Name: RG 108
Brand: Tocris Bioscience
SKU: 3295
Rating: 5 (2 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3295

Citations (3)

(2)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Non-nucleoside DNA methyltransferase inhibitor
Chemical Name:	N-Phthalyl-L-tryptophan
Purity:	≥99% (HPLC)
Molecular Weight:	334.33

View Full Technical Details for RG 108

Biological Activity for RG 108

RG 108 is a non-nucleoside DNA methyltransferase inhibitor that blocks the enzyme active site. Inhibits DNA methylation in human cancer cell lines in vitro without detectable toxicity. Demethylates and reactivates epigenetically silenced tumor suppressor genes. Enhances the efficiency of induced pluripotent stem cell (iPS) generation.

Compound Libraries for RG 108

RG 108 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for RG 108

M. Wt	334.33
Formula	C₁₉H₁₄N₂O₄
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	48208-26-0
PubChem ID	702558
InChi Key	HPTXLHAHLXOAKV-INIZCTEOSA-N
Smiles	O=C(C(C=CC=C4)=C4C3=O)N3[C@H](C(O)=O)CC2=CNC1=CC=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RG 108

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	33.43	100
	ethanol	33.43	100

Preparing Stock Solutions for RG 108

The following data is based on the product molecular weight 334.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.99 mL	14.96 mL	29.91 mL
5 mM	0.60 mL	2.99 mL	5.98 mL
10 mM	0.30 mL	1.50 mL	2.99 mL
50 mM	0.06 mL	0.30 mL	0.60 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for RG 108

References are publications that support the biological activity of the product.

Stresemann Functional diversity of DNA methyltransferase inhibitors in human cancer cell lines. Cancer Res. 2006 PMID: 16510601
Schirrmacher Synthesis and in vitro evaluation of biotinylated RG108: a high affinity compound for studying binding interactions with human DNA methyltransferases. Bioconjug.Chem. 2006 PMID: 16536454
Brueckner Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of DNA methyltransferase. Cancer Res. 2005 PMID: 16024632

Citations for RG 108 (3)

Showing 1 - 3 of 3 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for RG 108 include:

Zhang et al. (2016) Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation. Cell Stem Cell. 18:653
PMID: 27133794
Kottakis et al. (2016) LKB1 loss links serine metabolism to DNA methylation and tumorigenesis. Nature. 539:390
PMID: 27799657
Nugent et al. (2015) Brain feminization requires active repression of masculinization via DNA methylation. PLoS One. 18:690
PMID: 25821913

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for RG 108 (2)

5 out of 5

2 customer ratings

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Highly stable

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Neural Stem Cells

Cells are treated with 10 µM RG 108

PMID: 27133794
We got expected effect on cells

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Primary pancreatic ductal epithelial cells

100 μM RG108

PMID: PMID: 27799657

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

Product Documents for RG 108

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.