PI 828

Name: PI 828
Brand: Tocris Bioscience
SKU: 2814
Rating: 5 (1 reviews)

Catalog # 2814 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (5)

COA / SDS

PI 3-kinase inhibitor, more potent than LY 294002 (Cat. No. 1130)

Key Product Details

Description:	PI 3-kinase inhibitor, more potent than LY 294002 (Cat. No. 1130)
Chemical Name:	2-(4-Morpholinyl)-8-(4-aminopheny)l-4H-1-benzopyran-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	322.36

View Full Technical Details for PI 828

Technical Data
Citations (5) / Reviews (1)

Biological Activity for PI 828

PI 828 is a PI 3-Kinase inhibitor (IC₅₀ values are 0.098, 0.183, 0.227 and 1.967 μM for p110β, p110α, p110δ and p110γ respectively) that displays higher potency than LY 294002 (Cat No. 1130). Can be immobilized onto a solid phase.

Technical Data for PI 828

M. Wt	322.36
Formula	C₁₉H₁₈N₂O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	942289-87-4
PubChem ID	25181195
InChi Key	WUKMIBOGGXMBAC-UHFFFAOYSA-N
Smiles	O=C2C=C(N3CCOCC3)OC1=C(C4=CC=C(N)C=C4)C=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PI 828

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	32.24	100

Preparing Stock Solutions for PI 828

The following data is based on the product molecular weight 322.36.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.10 mL	15.51 mL	31.02 mL
5 mM	0.62 mL	3.10 mL	6.20 mL
10 mM	0.31 mL	1.55 mL	3.10 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

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Reconstitution Calculator

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References for PI 828

References are publications that support the biological activity of the product.

Gharbi Exploring the specificity of the PI3K family inhibitor LY294002. Biochem.J. 2007 PMID: 17302559

Citations for PI 828 (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for PI 828 include:

Liddell et al. (2016) Pyrrolidine dithiocarbamate activates the Nrf2 pathway in astrocytes. J Neuroinflammation. 13:49
PMID: 26920699
Rogers and Gahring (2015) Upregulation of Nicotinic Acetylcholine Receptor α4+β2 through a Ligand-Independent PI3Kβ Mechanism That Is Enhanced by TNFα and the Jak2/p38Mapk Pathways. J Neurosci. 10:e0143319
PMID: 26619345
Moser et al. (2014) Functional kinomics identifies candidate therapeutic targets in head and neck cancer. Clin Cancer Res. 20:4274
PMID: 25125259
Grassin-Delyle et al. (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res. 14:134
PMID: 24266887
Li et al. (2011) TRPV4-mediated calcium influx into human bronchial epithelia upon exposure to diesel exhaust particles. Environ Health Perspect. 119:784
PMID: 21245013

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Treat PI828 for HEK293 (293) cell line stably co-transfected with rat nAChR subunits alpha 4 and beta 2 are well characterized

Name: Wen Ding

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: 293

The HEK293 (293) cell line stably co-transfected with rat nAChR subunits alpha 4 and beta 2 are well characterized, and they were maintained as described previously. This cell line also expresses TNFR1 (only weak expression of TNFR2 is detected) and no additional human nAChR subunits or acetylcholine synthetic enzymes are detected. As before PI 828 (20 nM) treatments were conducted 48 hours after cell plating

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