Activation of NMDA receptors in outside-out patches
Assay Type: In Vitro
Cell Line/Tissue: Hippocampus
Mixture of 100 microM of glycine + 100 microM NMDA was used to evoke an NMDA receptor response without contamination from other glutamate receptors in outside-out patched excised from hippocampal neurons. NMDA displayed a clear agonist effect, as expected. Easily soluble, no problem if stored in frozen aliquots for more than half a year.
Should be used instead of glutamate for selective activation of NMDA receptors