Nimbolide
Tocris Bioscience, part of Bio-Techne | Catalog # 7289
RNF114 inhibitor
Product Description
Nimbolide inhibits auto-ubiquitination of the E3 ligase RNF114 and p21 ubiquitination in vitro. This compound impairs proliferation of triple-negative breast cancer cell lines. Nimbolide also induces apoptotic cell death in a Waldenströms macroglobulinemia cell line (BCWM1) and inhibits tumor growth in a xenograft model of Waldenströms macroglobulinemia.Nimbolide can be used for the development of Degraders that utilize RNF114 as an E3 ligase for Targeted Protein Degradation.
Product Specifications
Molecular Weight
466.53
Formula
C27H30O7
Storage
Store at -20°C
Purity
≥97% (HPLC)
Chemical Name
(2aR,5aR,6S,6aR,8R,9aR,10aS,10bR,10cR)-8-(3-Furanyl)-2a,5a,6,6a,8,9,9a,10a,10b,10c-decahydro-2a,5a,6a,7-tetramethyl-2,5-dioxo-2H,5H-cyclopenta[d']naphthodifuran-6-acetic acid methyl ester
CAS Number
25990-37-8
PubChem ID
12313376
InChI Key
JZIQWNPPBKFOPT-LSYMHUITSA-N
SMILES
[H][C@@]12C[C@@H](C3=COC=C3)C(C)=C1[C@]4([C@@H]([C@]5(C(C=C[C@]6(C(O[C@@]([C@]4(O2)[H])([C@]56[H])[H])=O)C)=O)C)CC(OC)=O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 46.65 | 100 |
Preparing Stock Solutions
for Nimbolide
The following data is based on the product molecular weight 466.53
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.14 mL | 10.72 mL | 21.43 mL |
| 5 mM | 0.43 mL | 2.14 mL | 4.29 mL |
| 10 mM | 0.21 mL | 1.07 mL | 2.14 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.43 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Spradin Harnessing the anti-cancer natural product nimbolide for targeted protein degradation. Nat.Chem.Biol. 2019 PMID: 31209351
- Tong A nimbolide-based kinase degrader preferentially degrades oncogenic BCR-ABL. ACS Chem.Biol. 2020 PMID: 32568522
- Chitta Nimbolide targets BCL2 and induces apoptosis in preclinical models of Waldenströms macroglobulinemia. Blood Cancer J. 2014 PMID: 25382610
Product Specific Notices for Nimbolide
For research use only
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