N-Acetylcysteine amide

Name: N -Acetylcysteine amide
Brand: Tocris Bioscience
SKU: 5619
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5619

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Citations (22)

COA / SDS

Glutathione (GSH) precursor; neuroprotective

Key Product Details

Description:	Glutathione (GSH) precursor; neuroprotective
Chemical Name:	(2R)-2-(Acetylamino)-3-mercaptopropanamide
Molecular Weight:	162.21

View Full Technical Details for N-Acetylcysteine amide

Technical Data
Citations / Reviews (1)

Biological Activity for N-Acetylcysteine amide

N-Acetylcysteine amide is a glutathione (GSH) precursor and cell-permeable antioxidant. N-Acetylcysteine amide replenishes intracellular Glutathione (GSH). N-Acetylcysteine amide directly reduces intracellular Glutathione disulfide (GSSG) to GSH without glutathione peroxidase. N-Acetylcysteine amide has anti-inflammatory activity through regulation of activation of NF-κB and HIF-1α, as well as modulation of reactive oxygen species. N-Acetylcysteine amide improves neuronal mitochondrial bioenergetics, reduces tissue damage and enhances functional recovery following spinal cord injury in rats. N-Acetylcysteine amide also enhances behavioral recovery in rats following traumatic brain injury. Neuroprotective. N-Acetylcysteine amide can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells (see protocol below).

For more information about how N-Acetylcysteine amide may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Technical Data for N-Acetylcysteine amide

M. Wt	162.21
Formula	C₅H₁₀N₂O₂S
Storage	Store at -20°C
CAS Number	38520-57-9
PubChem ID	10176265
InChi Key	UJCHIZDEQZMODR-BYPYZUCNSA-N
Smiles	SC[C@H](NC(C)=O)C(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for N-Acetylcysteine amide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	16.22	100
	DMSO	16.22	100

Preparing Stock Solutions for N-Acetylcysteine amide

The following data is based on the product molecular weight 162.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.16 mL	30.82 mL	61.65 mL
5 mM	1.23 mL	6.16 mL	12.33 mL
10 mM	0.62 mL	3.08 mL	6.16 mL
50 mM	0.12 mL	0.62 mL	1.23 mL

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Reconstitution Calculator

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References for N-Acetylcysteine amide

References are publications that support the biological activity of the product.

Pandya N-acetylcysteine amide confers neuroprotection, improves bioenergetics and behavioral outcome following TBI. Exp.Neurol. 2014 PMID: 24792639
Tu The anti-inflammatory and anti-oxidant mechanisms of the Keap1/Nrf2/ARE signaling pathway in chronic diseases. Aging Dis 2019 PMID: 31165007
Patel N-acetylcysteine amide preserves mitochondrial bioenergetics and improves functional recovery following spinal trauma. Exp.Neurol. 2014 PMID: 24805071