N-Acetylcysteine amide is a glutathione (GSH) precursor and cell-permeable antioxidant. N-Acetylcysteine amide replenishes intracellular Glutathione (GSH). N-Acetylcysteine amide directly reduces intracellular Glutathione disulfide (GSSG) to GSH without glutathione peroxidase. N-Acetylcysteine amide has anti-inflammatory activity through regulation of activation of NF-κB and HIF-1α, as well as modulation of reactive oxygen species. N-Acetylcysteine amide improves neuronal mitochondrial bioenergetics, reduces tissue damage and enhances functional recovery following spinal cord injury in rats. N-Acetylcysteine amide also enhances behavioral recovery in rats following traumatic brain injury. Neuroprotective. N-Acetylcysteine amide can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells (see protocol below).
For more information about how N-Acetylcysteine amide may be used, see our protocol: 3D Culture of Lung Alveolar Cells
