ML 323

Name: ML 323
Brand: Tocris Bioscience
SKU: 6435

Tocris Bioscience, part of Bio-Techne | Catalog # 6435

Citations (5)

COA / SDS

Potent and selective USP1-UAF1 allosteric inhibitor

Key Product Details

Description:	Potent and selective USP1-UAF1 allosteric inhibitor
Chemical Name:	5-Methyl-2-[2-(1-methylethyl)phenyl]-N-[[4-(1H-1,2,3-triazol-1-yl)phenyl]methyl]-4-pyrimidinamine
Purity:	≥98% (HPLC)
Molecular Weight:	384.48

View Full Technical Details for ML 323

Technical Data
Citations

Biological Activity for ML 323

ML 323 is a potent and selective allosteric inhibitor of USP1-UAF1 deubiquitinase complex (IC₅₀ = 76 nM). Exhibits selectivity for USP1-UAF1 over a panel of enzymes, including other deuquitinating enzymes. Inhibits deubiquitination of PCNA and FANCD2 and sensitizes cisplatin-resistant H596 cells to cell death induced by cisplatin (Cat. No. 2251) in vitro.

Compound Libraries for ML 323

ML 323 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 323

M. Wt	384.48
Formula	C₂₃H₂₄N₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1572414-83-5
PubChem ID	60167849
InChi Key	VUIRVWPJNKZOSS-UHFFFAOYSA-N
Smiles	CC(C)C1=C(C=CC=C1)C1=NC=C(C)C(NCC2=CC=C(C=C2)N2C=CN=N2)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 323

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	38.45	100
	ethanol	38.45	100

Preparing Stock Solutions for ML 323

The following data is based on the product molecular weight 384.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.60 mL	13.00 mL	26.01 mL
5 mM	0.52 mL	2.60 mL	5.20 mL
10 mM	0.26 mL	1.30 mL	2.60 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

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References for ML 323

References are publications that support the biological activity of the product.

Liang A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat.Chem.Biol. 2014 PMID: 24531842