ML 297

Name: ML 297
Brand: Tocris Bioscience
SKU: 5380
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 5380

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Key Product Details

Description:	Selective K_ir3.1/3.2 (GIRK1/2) channel activator
Chemical Name:	N-(3,4-Difluorophenyl)-N'-(3-methyl-1-phenyl-1H-pyrazol-5-yl)urea
Purity:	≥99% (HPLC)
Molecular Weight:	328.32

View Full Technical Details for ML 297

Biological Activity for ML 297

ML 297 is a selective K_ir3.1/3.2 (GIRK1/2) channel activator (IC₅₀ values are 160, 887 and 914 nM for GIRK1/2, GIRK1/4 and GIRK1/3 respectively). Exhibits no effect on GIRK2, GIRK2/3, K_ir2.1 and K_v7.4 channels, and has minimal effect on a panel of other ion channels, receptors and transporters. Prevents PTZ-induced epileptic seizures in mice. Brain penetrant.

Licensing Information

Sold under license from Vanderbilt University

Compound Libraries for ML 297

ML 297 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 297

M. Wt	328.32
Formula	C₁₇H₁₄F₂N₄O
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	1443246-62-5
PubChem ID	56642816
InChi Key	IEKSMUSSYJUQMY-UHFFFAOYSA-N
Smiles	O=C(NC2=CC(C)=NN2C3=CC=CC=C3)NC1=CC(F)=C(F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 297

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	32.83	100
	ethanol	16.42	50

Preparing Stock Solutions for ML 297

The following data is based on the product molecular weight 328.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.05 mL	15.23 mL	30.46 mL
5 mM	0.61 mL	3.05 mL	6.09 mL
10 mM	0.30 mL	1.52 mL	3.05 mL
50 mM	0.06 mL	0.30 mL	0.61 mL

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References for ML 297

References are publications that support the biological activity of the product.

Kaufmann ML297 (VU0456810), the first potent and selective activator of the GIRK potassium channel, displays antiepileptic properties in mice. ACS Chem.Neurosci. 2013 PMID: 23730969
Wen Discovery of 'molecular switches' within a GIRK activator scaffold that afford selective GIRK inhibitors. Bioorg.Med.Chem.Lett. 2013 PMID: 23838260

Citations for ML 297

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ML 297 worked ideally and the data obtained with the product was consistent throughout the study

Name: Anonymous

Species: Human

Assay Type: In Vitro

ML 297 and ML 277 was used on our University labs to understand potassium channel activation percentage at increasing concentrations. ML 297 worked ideally and the data obtained with the product was consistent throughout the experiment.

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