Methotrexate
Tocris Bioscience, part of Bio-Techne | Catalog # 1230
Cytotoxic agent
Product Description
Methotrexate is a cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.Product Specifications
Molecular Weight
454.44
Formula
C20H22N8O5
Storage
Store at RT
Purity
≥98% (HPLC)
Chemical Name
N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid
CAS Number
59-05-2
PubChem ID
126941
InChI Key
FBOZXECLQNJBKD-ZDUSSCGKSA-N
SMILES
O=C(N[C@H]([C@](O)=O)CCC(O)=O)C(C=C3)=CC=C3N(CC2=NC1=C(N)N=C(N)N=C1N=C2)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 100 |
Preparing Stock Solutions
for Methotrexate
The following data is based on the product molecular weight 454.44
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.20 mL | 11.00 mL | 22.01 mL |
| 5 mM | 0.44 mL | 2.20 mL | 4.40 mL |
| 10 mM | 0.22 mL | 1.10 mL | 2.20 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Winter-Vann Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotr. Proc.Natl.Acad.Sci.U.S.A. 2003 PMID: 12750467
- Chu Mechanism of thymidylate synthase inhibition by methotr. in human neoplastic cell lines and normal human myeloid progenitor cells. J.Biol.Chem. 1990 PMID: 2341391
- Jolivet The pharmacology and clinical use of methotr. N.Engl.J.Med. 1983 PMID: 6353235
- Allegra Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotr. J.Biol.Chem. 1987 PMID: 2443493
Product Specific Notices for Methotrexate
For research use only
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov
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