JB 300
Tocris Bioscience, part of Bio-Techne | Catalog # 7837
Aurora A Degrader (PROTAC®)
Product Description
JB 300 is a potent and selective Aurora A Degrader (PROTAC®; DC50 = 30 nM and Dmax = 78%). It comprises a cereblon E3-ligase ligand Thalidomide (Cat. No. 0652) joined by a PEG2 linker to the Aurora A inhibitor MK-5108. The compound depletes Aurora A in MV4-11 cell lines and exhibits no degradation of Aurora B at 10 µM. JB 300 shows dose-dependent cytotoxicity in AML cells.Aurora A antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF3295 and NBP1-51843.
PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
Licensing Information
Sold under license from University of WürzburgProduct Specifications
Molecular Weight
906.38
Formula
C43H45ClFN7O10S
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
trans-4-(3-Chloro-2-fluorophenoxy)-N-(2-(2-(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethoxy)ethoxy)ethyl)-1-((6-(thiazol-2-ylamino)pyridin-2-yl)methyl)cyclohexane-1-carboxamide
CAS Number
3038446-90-8
InChI Key
LAZHTWDJEQSMOY-NKEKBGIASA-N
SMILES
O=C([C@@]1(CC2=CC=CC(NC3=NC=CS3)=N2)CC[C@H](OC4=C(F)C(Cl)=CC=C4)CC1)NCCOCCOCCNC(COC5=C6C(N(C7CCC(NC7=O)=O)C(C6=CC=C5)=O)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 18.13 | 20 |
Preparing Stock Solutions
for JB 300
The following data is based on the product molecular weight 906.38
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 5.52 mL | 27.58 mL | 55.16 mL |
| 1 mM | 1.10 mL | 5.52 mL | 11.03 mL |
| 2 mM | 0.55 mL | 2.76 mL | 5.52 mL |
| 10 mM | 0.11 mL | 0.55 mL | 1.10 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Bozolovic Novel, highly potent PROTACs targeting AURORA-A kinase. Curr.Res.Chem.Biol.
- Adhikari PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase. Nat.Chem.Biol. 2020 PMID: 32989298
Product Specific Notices for JB 300
For research use only
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