IDE 1

Name: IDE 1
Brand: Tocris Bioscience
SKU: 4015

Tocris Bioscience, part of Bio-Techne | Catalog # 4015

Citations (8)

COA / SDS

Induces definitive endoderm formation in mouse and human ESCs

Key Product Details

Description:	Induces definitive endoderm formation in mouse and human ESCs
Chemical Name:	1-[2-[(2-Carboxyphenyl)methylene]hydrazide]heptanoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	306.31

View Full Technical Details for IDE 1

Technical Data
Citations

Biological Activity for IDE 1

IDE 1 is an induces definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC₅₀ = 125 nM). Thought to activate the TGF-β signaling pathway; induces Smad2 phosphorylation and increases levels of Nodal expression.

This compound is mixture of E/Z isomers.

Licensing Information

Sold for research purpose under license from Harvard University.

Technical Data for IDE 1

M. Wt	306.31
Formula	C₁₅H₁₈N₂O₅
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1160927-48-9
PubChem ID	66974738
InChi Key	ABKJCDILEUEJSH-UHFFFAOYSA-N
Smiles	OC(=O)CCCCCC(=O)NN=CC1=CC=CC=C1C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for IDE 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	2eq. NaOH	30.63	100
	DMSO	30.63	100

Preparing Stock Solutions for IDE 1

The following data is based on the product molecular weight 306.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.26 mL	16.32 mL	32.65 mL
5 mM	0.65 mL	3.26 mL	6.53 mL
10 mM	0.33 mL	1.63 mL	3.26 mL
50 mM	0.07 mL	0.33 mL	0.65 mL

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References for IDE 1

References are publications that support the biological activity of the product.

Zaret Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 2009 PMID: 19427285
Filby Stimulation of AVNA/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2011 PMID: 21463501
Borowiak Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 2009 PMID: 19341624