IBG3
Tocris Bioscience, part of Bio-Techne | Catalog # 8129
Potent molecular glue Degrader of BET proteins BRD4 and BRD2
Key Product Details
Description
Potent molecular glue Degrader of BET proteins BRD4 and BRD2
Product Description
IBG3 is a very potent JQ1-containing molecular glue Degrader of BET proteins BRD4 and BRD2 (DC50 values are 6.7 pM and 8.6 pM respectively). Induces proximity between BRD4 and DCAF16 to form a ternary complex (EC50 = 32 nM).Scientific Data Images for IBG3
Published vs Bio-Techne batch comparison for IBG3.
Endogenous HiBiT-BRD4 HEK293 cells were plated at a density of 20,000 cell per well in 100 µL of DMEM (10% FBS) in a white-walled 96 well plate and left overnight to adhere at 37 ºC, 5% CO2. Cells were incubated in 50 µL of 10 nM-0.1 pM of IBG3 or vehicle control (DMSO) in Optimem (10% FBS) and left for 4 hours. Cells were then treated with HiBIT Lytic assay reagent– and plates were then read on a BMG Pherastar plate reader for luminescence detection. Data kindly provided by the Ciulli laboratory, University of Dundee.Product Specifications
Molecular Weight
976.23
Formula
C54H57N9O5S2
Storage
Store at -20°C
Chemical Name
tert-Butyl 2-((S)-4-(4-((4'-((S)-6-(2-(tert-butoxy)-2-oxoethyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-4-yl)-[1,1'-biphenyl]-4-carboxamido)methyl)phenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetate
PubChem ID
171037958
InChI Key
MRIUQCAXSFYTQZ-YATWDLPUSA-N
SMILES
CC1=C(SC2=C1C(C3=CC=C(C=C3)C4=CC=C(C=C4)C(NCC5=CC=C(C6=N[C@H](C7=NN=C(N7C8=C6C(C)=C(S8)C)C)CC(OC(C)(C)C)=O)C=C5)=O)=N[C@H](C9=NN=C(N92)C)CC(OC(C)(C)C)=O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 97.62 | 100 |
Preparing Stock Solutions
for IBG3
The following data is based on the product molecular weight 976.23
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.02 mL | 5.12 mL | 10.24 mL |
| 5 mM | 0.20 mL | 1.02 mL | 2.05 mL |
| 10 mM | 0.10 mL | 0.51 mL | 1.02 mL |
| 50 mM | 0.02 mL | 0.10 mL | 0.20 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Hsia Targeted protein degradation via intramolecular bivalent glues. Nature 2024 PMID: 38383787
Product Specific Notices for IBG3
For research use only
Loading...
Loading...