endo-IWR 1

Name: endo -IWR 1
Brand: Tocris Bioscience
SKU: 3532

Catalog # 3532 | Tocris Bioscience a Bio-Techne Brand

Citations (26)

COA / SDS

Wnt/β-catenin signaling inhibitor; axin stabilizer

Key Product Details

Description:	Wnt/β-catenin signaling inhibitor; axin stabilizer
Chemical Name:	rel-4-[(3aR,4S,7R,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide
Purity:	≥98% (HPLC)
Molecular Weight:	409.44

View Full Technical Details for endo-IWR 1

Technical Data
Citations (26) / Reviews

Biological Activity for endo-IWR 1

endo-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells and may be used in protocols for the generation of retinal pigment epithelial cells from hPSCs.

Negative Control also available.

endo-IWR 1 synthesized to Ancillary Material Grade also available.

For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Compound Libraries for endo-IWR 1

endo-IWR 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for endo-IWR 1

M. Wt	409.44
Formula	C₂₅H₁₉N₃O₃
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	1127442-82-3
PubChem ID	90488854
InChi Key	ZGSXEXBYLJIOGF-ALFLXDJESA-N
Smiles	[H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for endo-IWR 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.94	100

Preparing Stock Solutions for endo-IWR 1

The following data is based on the product molecular weight 409.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.44 mL	12.21 mL	24.42 mL
5 mM	0.49 mL	2.44 mL	4.88 mL
10 mM	0.24 mL	1.22 mL	2.44 mL
50 mM	0.05 mL	0.24 mL	0.49 mL

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References for endo-IWR 1

References are publications that support the biological activity of the product.

Surendran An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 2022 PMID: 36386870
Reichman Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 2018 PMID: 29217753
Lancaster Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 2015 PMID: 25188634
Kadoshima Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 2013 PMID: 24277810
Lu Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 2009 PMID: 19410457
Chen Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 2008 PMID: 19125156

Citations for endo-IWR 1 (26)

Showing 1 - 10 of 26 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for endo-IWR 1 include:

Erika et al. (2023) Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling. Sci Rep. 13:3186
PMID: 36823310
Capeling et al. (2022) Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep.. 38:110379
PMID: 35172130
Martin et al. (2022) A specialized bone marrow microenvironment for fetal haematopoiesis. Nat Commun. 13:1327
PMID: 35288551
Alexandre P et al. (2022) An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates. Elife. 11
PMID: 35536602
Min et al. (2022) Comparison of chromatin accessibility landscapes during early development of prefrontal cortex between rhesus macaque and human. Nat Commun. 13:3883
PMID: 35794099
W Hayes et al. (2022) DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3. JCI Insight. 7
PMID: 34941574
Jia et al. (2022) Selective activation and down-regulation of Trk receptors by neurotrophins in human neurons co-expressing TrkB and TrkC. J Neurochem. 161:463-477
PMID: 35536742
Timothy C et al. (2022) Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells. Elife. 11
PMID: 36129169
Esra et al. (2021) Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Methods Mol Biol. 2520:161-170
PMID: 34845657
Eunjeong et al. (2020) TAZ/Wnt-β-catenin/c-MYC axis regulates cystogenesis in polycystic kidney disease. Proc Natl Acad Sci U S A. 117:29001-29012
PMID: 33122431
Alon et al. (2020) Electrophysiologic Characterization of Developing Human Embryonic Stem Cell-Derived Photoreceptor Precursors. Invest Ophthalmol Vis Sci. 61:44
PMID: 32991686
Geoffrey et al. (2020) Tissue-Specific Regulation of the Wnt/β-Catenin Pathway by PAGE4 Inhibition of Tankyrase. Cell Rep. 32:107922
PMID: 32698014
Le et al. (2020) Vascularized human cortical organoids (vOrganoids) model cortical development in vivo. PLoS Biol. 18:e3000705
PMID: 32401820
Amos et al. (2020) SEM/FIB Imaging for Studying Neural Interfaces. Dev Neurobiol. 80:305-315
PMID: 31228876
Gao et al. (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Bio. 21:687
PMID: 31160711
Garcia et al. (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci. 13:669
PMID: 31316341
Yamoah et al. (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine. 13:6073
PMID: 30323594
Suzuki et al. (2018) The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Sci Rep. 8:15057
PMID: 30305648
Ali H et al. (2018) hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate. Cell Stem Cell. 23:60-73.e6
PMID: 29937203
Kishimoto (2017) Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells. PLoS One. 12(7):e0182051
PMID: 28750046
Kennedy et al. (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A. 113:3545
PMID: 26969725
Latos et al. (2015) Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun. 6:7776
PMID: 26206133
Lindström et al. (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Mol Cell Biol. 3:e04000
PMID: 25647637
Suzuki et al. (2015) WNT/β-Catenin Signaling Regulates Multiple Steps of Myogenesis by Regulating Step-Specific Targets. Development. 35:1763
PMID: 25755281
Iwata et al. (2014) TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. Nat Protoc. 141:909
PMID: 24496627
Cajal et al. (2014) A conserved role for non-neural ectoderm cells in early neural development. Development. 141:4127
PMID: 25273086

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