endo-IWR 1

Name: endo -IWR 1
Brand: Tocris Bioscience
SKU: 3532

Tocris Bioscience, part of Bio-Techne | Catalog # 3532

Citations (47)

COA / SDS

Wnt/β-catenin signaling inhibitor; axin stabilizer

Key Product Details

Description:	Wnt/β-catenin signaling inhibitor; axin stabilizer
Chemical Name:	rel-4-[(3aR,4S,7R,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide
Purity:	≥98% (HPLC)
Molecular Weight:	409.44

View Full Technical Details for endo-IWR 1

Technical Data
Citations

Biological Activity for endo-IWR 1

endo-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells and may be used in protocols for the generation of retinal pigment epithelial cells from hPSCs.

Negative Control also available.

endo-IWR 1 synthesized to Ancillary Material Grade also available.

For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Compound Libraries for endo-IWR 1

endo-IWR 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for endo-IWR 1

M. Wt	409.44
Formula	C₂₅H₁₉N₃O₃
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	1127442-82-3
PubChem ID	90488854
InChi Key	ZGSXEXBYLJIOGF-ALFLXDJESA-N
Smiles	[H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for endo-IWR 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.94	100

Preparing Stock Solutions for endo-IWR 1

The following data is based on the product molecular weight 409.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.44 mL	12.21 mL	24.42 mL
5 mM	0.49 mL	2.44 mL	4.88 mL
10 mM	0.24 mL	1.22 mL	2.44 mL
50 mM	0.05 mL	0.24 mL	0.49 mL

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References for endo-IWR 1

References are publications that support the biological activity of the product.

Surendran An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 2022 PMID: 36386870
Reichman Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 2018 PMID: 29217753
Lancaster Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 2015 PMID: 25188634
Kadoshima Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 2013 PMID: 24277810
Chen Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 2008 PMID: 19125156
Lu Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 2009 PMID: 19410457