EB 47

Name: EB 47
Brand: Tocris Bioscience
SKU: 4140

Tocris Bioscience a Bio-Techne Brand

Catalog # 4140

Citations (3)

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Key Product Details

Description:	Potent PARP-1 inhibitor
Chemical Name:	5'-Deoxy-5'-[4-[2-[(2,3-Dihydro-1-oxo-1H-isoindol-4-yl)amino]-2-oxoethyl]-1-piperazinyl]-5'-oxoadenosine dihydrochloride
Purity:	≥99% (HPLC)
Molecular Weight:	610.45

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Biological Activity for EB 47

EB 47 is a potent inhibitor of PARP-1 (IC₅₀ = 45 nM). Reduces infarct volume in both a rat transient middle cerebral arterial occlusion model and a cardiac reperfusion model.

Technical Data for EB 47

M. Wt	610.45
Formula	C₂₄H₂₇N₉O₆.2HCl
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	1190332-25-2
PubChem ID	90488915
InChi Key	VVMQSDIMNDTMII-MYXHFVDASA-N
Smiles	NC1=C2N=CN([C@@H]3O[C@@H]([C@@H](O)[C@H]3O)C(N4CCN(CC(NC5=C6CNC(C6=CC=C5)=O)=O)CC4)=O)C2=NC=N1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for EB 47

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	3.05	5 with gentle warming
	DMSO	30.52	50

Preparing Stock Solutions for EB 47

The following data is based on the product molecular weight 610.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.28 mL	16.38 mL	32.76 mL
2.5 mM	0.66 mL	3.28 mL	6.55 mL
5 mM	0.33 mL	1.64 mL	3.28 mL
25 mM	0.07 mL	0.33 mL	0.66 mL

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References for EB 47

References are publications that support the biological activity of the product.

Ferraris Evolution of poly(ADP-ribose) polymerase-1 (PARP-1) inhibitors. From concept to clinic. J.Med.Chem. 2010 PMID: 20364863
Gaymes Inhibitors of poly ADP-ribose polymerase (PARP) induce apoptosis of myeloid leukemic cells: potential for therapy of myeloid leukemia and myelodysplastic syndromes. Haematologica 2009 PMID: 19407318
Jagtap The discovery and synthesis of novel adenosine substituted 2,3-dihydro-1H-isoindol-1-ones: potent inhibitors of poly(ADP-ribose) polymerase-1 (PARP-1). Bioorg.Med.Chem.Lett. 2004 PMID: 14684303

Citations for EB 47 (3)

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Citations are publications that use Bio-Techne products. Selected citations for EB 47 include:

Rom et al. (2016) PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier Journal of Neuroinflammation. 13:254
PMID: 27677851
Rom et al. (2015) Poly(ADP-ribose) polymerase-1 inhibition in brain endothelium protects the blood-brain barrier under physiologic and neuroinflammatory conditions. Front Microbiol. 35:28
PMID: 25248836
Rom et al. (2015) The dual action of poly(ADP-ribose) polymerase -1 (PARP-1) inhibition in HIV-1 infection: HIV-1 LTR inhibition and diminution in Rho GTPase activity. Dev Biol. 6:878
PMID: 26379653

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