Diphenyleneiodonium chloride is a GPR3 agonist (EC₅₀ = 1 μM); activates adenylate cyclase through GPR3 but not GPR6 or GPR12. Also induces Ca²⁺ mobilization and β-arrestin receptor internalization. Binds strongly to flavoproteins; inhibits several enzymes, including NO synthase, NADPH oxidases and NADPH cytochrome P450 oxidoreductase. Also inhibits platelet aggregation.

Product Specifications

Molecular Weight

314.55

Formula

C₁₂H₈ClI

Storage

Desiccate at -20°C

Purity

≥98% (HPLC)

Chemical Name

[1,1'-Biphenyl]-2,2'-diyliodonium chloride

CAS Number

4673-26-1

PubChem ID

2733504

InChI Key

FCFZKAVCDNTYID-UHFFFAOYSA-M

SMILES

[Cl-].c1c2c3ccccc3[i+]c2ccc1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	3.14	10

Preparing Stock Solutions for Diphenyleneiodonium chloride

The following data is based on the product molecular weight 314.55

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.79 mL	158.96 mL	317.91 mL
0.5 mM	6.36 mL	31.79 mL	63.58 mL
1 mM	3.18 mL	15.90 mL	31.79 mL
5 mM	0.64 mL	3.18 mL	6.36 mL

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Background References

References are publications that support the biological activity of the product.

Ye Identification of a novel small-molecule agonist for human G protein-coupled receptor 3. J.Pharmacol.Exp.Ther. 2014 PMID: 24633425
Tew Inhibition of cytochrome P450 reductase by the diphenyliodonium cation. Kinetic analysis and covalent modifications. Biochemistry 1993 PMID: 8399148
Wang Inhibitory actions of diphenyleneiodonium dependent vasodilations in vitro and in vivo. Br.J.Pharmacol. 1993 PMID: 7507779
Yea Purification and some properties of the 45 kDa diphenylene iodonium-binding flavoprotein of neutrophil NADPH oxidase. Biochem.J. 1990 PMID: 2154184
Stuehr Inhibition of macrophage and endothelial cell nitric oxide synthase by diphenyleneiodonium and its analogs. FASEB J. 1991 PMID: 1703974

Diphenyleneiodonium chloride

Tocris Bioscience, part of Bio-Techne | Catalog # 0504

Key Product Details

Description

Alternative Names

View all Additional Nitric Oxide Compounds »

Product Description