Cyclosporin A

Name: Cyclosporin A
Brand: Tocris Bioscience
SKU: 1101
Rating: 4 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 1101

Citations (25)

(1)

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Key Product Details

Description:	Calcineurin inhibitor
Alternative Names:	Ciclosporin A
Purity:	≥99% (HPLC)
Molecular Weight:	1202.63

View Full Technical Details for Cyclosporin A

Biological Activity for Cyclosporin A

Cyclosporin A is an immunosuppressant; simultaneously binds calcineurin and cyclophilin and inhibits phosphatase activity of calcineurin (IC₅₀ = 5 nM). Additionally inhibits formation and opening of the mitochondrial permeability transition pore (MPTP). Also inhibits coronavirus replication in vitro. Enhances lentirival transduction of bone marrow-derived CD34⁺ cells and is additive with Prostaglandin E₂ (Cat. No. 2296).

Compound Libraries for Cyclosporin A

Cyclosporin A is also offered as part of the Tocriscreen Antiviral Library, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Cyclosporin A

M. Wt	1202.63
Formula	C₆₂H₁₁₁N₁₁O₁₂
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	59865-13-3
PubChem ID	2909
InChi Key	PMATZTZNYRCHOR-CGLBZJNRSA-N
Smiles	[H][C@@]1(C(C)C)NC([C@@H](N(C(CN(C([C@@H](NC([C@]([C@@H]([C@@H](C/C=C/C)C)O)(N(C([C@](C(C)C)(N(C([C@@H](N(C([C@](CC(C)C)(N(C([C@H](NC([C@@H](NC([C@@H](N(C1=O)C)CC(C)C)=O)C)=O)C)=O)C)[H])=O)C)CC(C)C)=O)C)[H])=O)C)[H])=O)CC)=O)C)=O)C)CC(C)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cyclosporin A

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	120.26	100
	ethanol	60.13	50

Preparing Stock Solutions for Cyclosporin A

The following data is based on the product molecular weight 1202.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.83 mL	4.16 mL	8.32 mL
5 mM	0.17 mL	0.83 mL	1.66 mL
10 mM	0.08 mL	0.42 mL	0.83 mL
50 mM	0.02 mL	0.08 mL	0.17 mL

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References for Cyclosporin A

References are publications that support the biological activity of the product.

Petrillo Assessing the impact of cyclosporin A on lentiviral transduction and preservation of human hematopoietic stem cells in clinically relevant ex vivo gene therapy settings. Hum.Gene.Ther. 2019 PMID: 31037976
Carbajo-Lozoya Human coronavirus NL63 replication is cyclophilin A-dependent and inhibited by non-immunosuppressive cyclosporine A-derivatives including Alisporivir. Virus Res. 2014 PMID: 24566223
de Wilde MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-α treatment. J.Gen.Virol. 2013 PMID: 23620378
de Wilde Cyclosporin A inhibits the replication of diverse coronaviruses. J.Gen.Virol. 2011 PMID: 21752960
Yan Cyclosporin-A potently induces highly cardiogenic progenitors from embryonic stem cells. Biochem.Biophys.Res.Commun. 2009 PMID: 19094963
Nakata Evidence that cyclosporin A and dexameth. inhibit allergic airway inflammation via suppression of interleukin-5 synthesis by T cells. Br.J.Pharmacol. 1998 PMID: 9723954
Brookemeier and Pfeiffer Inhibition of the mitochondrial permeability transition pore by cyclosporin A during long time frame experiments: relationship between pore opening and the activity of mitochondrial phospholipases. Biochemistry 1995 PMID: 8845372
Fruman Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A. Proc.Natl.Acad.Sci.U.S.A. 1992 PMID: 1373887

Citations for Cyclosporin A (25)

Showing 1 - 10 of 25 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Cyclosporin A include:

Lopez et al. (2022) Ketamine exerts its sustained antidepressant effects via cell-type-specific regulation of Kcnq2. Neuron. 110:2283
PMID: 35649415
Battefeld et al. (2019) High-Frequency Microdomain Ca²⁺ Transients and Waves during Early Myelin Internode Remodeling. Cell Rep. 26:182
PMID: 30605675
Bhosale and Duchen (2019) Investigating the Mitochondrial Permeability Transition Pore in Disease Phenotypes and Drug Screening. Curr Protoc Pharmacol. :e59
PMID: 31081999
Puri et al. (2019) Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts. Nat Commun. 10:3645
PMID: 31409786
Panja et al. (2019) Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity. Neuropathol Appl Neurobiol. 45:141
PMID: 29679372
P?rez et al. (2018) Mitochondrial permeability transition pore contributes to mitochondrial dysfunction in fibroblasts of patients with sporadic Alzheimer's disease. Redox Biol. 19:290
PMID: 30199818
Burdyga et al. (2018) Phosphatases control PKA-dependent functional microdomains at the outer mitochondrial membrane. Proc Natl Acad Sci U S A.. 115(28):E6497
PMID: 29941564
Agarwal et al. (2017) Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes. Neuron. 93:587
PMID: 28132831
Miyamoto et al. (2016) Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-β-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity. Cardiorenal Med. 291:1719
PMID: 26589795
Cui et al. (2016) Endocannabinoid dynamics gate spike-timing dependent depression and potentiation. Elife. 5:e13185
PMID: 26920222
Palmqvist et al. (2016) A human and animal model-based approach to investigating the anti-inflammatory profile and potential of the 5-HT2B receptor antagonist AM1030. J Inflamm (Lond). 13:20
PMID: 27340371
O'Donnell et al. (2016) Transient Oxygen/Glucose Deprivation Causes a Delayed Loss of Mitochondria and Increases Spontaneous Calcium Signaling in Astrocytic Processes. J Neurosci. 36:7109
PMID: 27383588
Piccart et al. (2015) Neurotensin Induces Presynaptic Depression of D2 DA Autoreceptor-Mediated Neurotransmission in Midbrain DArgic Neurons. J Neurosci. 35:11144
PMID: 26245975
Draganov et al. (2015) Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via iverm. induces a non-apoptotic and inflammatory form of cancer cell death. J Neurosci. 5:16222
PMID: 26552848
Liu et al. (2015) Hyperphosphate-Induced Myocardial Hypertrophy through the GATA-4/NFAT-3 Signaling Pathway Is Attenuated by ERK Inhibitor Treatment. Exp Eye Res. 5:79
PMID: 25999956
Faralli et al. (2015) NFATc1 activity regulates the expression of myocilin induced by dexameth. J Biol Chem. 130:42614
PMID: 25450062
Yu et al. (2014) Differential effects of the Toll-like receptor 2 agonists, PGN and Pam3CSK4 on anti-IgE induced human mast cell activation. PLoS One. 9:e112989
PMID: 25398056
Gray et al. (2014) Inhibitory interactions between phosphorylation sites in the C terminus of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor GluA1 subunits. Mol Immunol. 289:14600
PMID: 24706758
Gross et al. (2013) Roles of endothelial nitric oxide synthase (eNOS) and mitochondrial permeability transition pore (MPTP) in epoxyeicosatrienoic acid (EET)-induced cardioprotection against infarction in intact rat hearts. J Mol Cell Cardiol. 59:20
PMID: 23419451
Pont et al. (2012) Oxytocin-stimulated NFAT transcriptional activation in human myometrial cells. Mol.Endocrinol.. 26:1743
PMID: 22902539
Li and Burrell (2011) Associative, bidirectional changes in neural signaling utilizing NMDA receptor- and endocannabinoid-dependent mechanisms. Learn Mem. 18:545
PMID: 21844187
George et al. (2011) Calcium-dependent phosphorylation regulates neuronal stability and plasticity in a highly precise pacemaker nucleus. J Neurophysiol. 106:319
PMID: 21525377
Ceglia et al. (2010) Signaling pathways controlling the phosphorylation state of WAVE1, a regulator of actin polymerization. J Neurochem. 114:182
PMID: 20403076
Grodzki et al. (2009) FcεRI-induced activation by low antigen concentrations results in nuclear signals in the absence of degranulation. PLoS One. 46:2539
PMID: 19540596
Belmeguenai and Hansel (2005) A role for protein phosphatases 1, 2A, and 2B in cerebellar long-term potentiation. J Biol Chem. 25:10768
PMID: 16291950

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Cyclosporin against synthetic immunosuppressant

Name: Anonymous

Species: Human

Used cyclosporin as a natural control to understand few other synthetic immunosuppressants.

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Product Documents for Cyclosporin A

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.