Cy5-Fuc Labeled sulfo-G2 (Cy5-sulfo G2)

Applications

• Used as a ligand for lectins and to study glycan protein interaction.
• Used as a substrate for various glycosidase and glycosyltransferases.
• Used as a substrate to study sulfatases.

Key Features:

• Excitation at 649 nm and emission at 671 nm.
• The fluorescent dye Cy5 is conjugated to the C6 position of the core-6 fucose.
• Can be separated on 15-17% SDS-PAGE and directly visualized as a single band through the red channel of a fluorescent imager.
• Linear response range for Cy5-labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.

Related Reagents

Labeled Glycans

• Cy5-Fuc Labeled M1N1f (GL301)
• Cy5-Fuc Labeled Glycan G2f (GL302)
• Cy5-Fuc Labeled N2f (GL304)
• Fluorescent Glycan Labeling and Detection

Click Chemistry

• GDP-Cy5-Fucose (ES301)
• CMP-Cy5-Sialic Acid (ES302)
• GDP-Cy3-Fucose (ES401)
• CMP-Cy3-Sialic Acid (ES402)

Enzymes and detection reagents

• Various sulfatases
• Various sialytransferases
• Various fucosyltransferases
• Various neuraminidases/sialidases

Sulfatases belong to a highly conserved family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. Among 18 human sulfatase genes identified, about 1/3 are found in lysosomes and serve to remove sulfate from glycosaminoglycans (GAGs), glycopeptides and glycolipids (GLs). The remaining sulfatases are found in microsomes, endoplasmic reticulum (ER), Golgi, at the cell surface, or secreted and are active against hydroxysteroids, heparin or unidentified substrates. Genetic deficiencies of individual sulfatases reveal the critical importance of these enzymes in the metabolism of specific forms of sulfate. Deficiencies of lysosomal sulfatases that act on GAGs and GLs account for mucopolysaccharidoses (MPS) and metachromatic leukodystrophy, respectively. Deficiencies in non-lysosomal sulfatases, arylsulfases C and E, cause X-linked ichthyosis (XLI) and chondrodysplasia punctata (CDPX), respectively.