Cy5-Fuc Labeled Glycan G2f

Applications

• Used as a ligand for lectins and to study glycan protein interaction.
• Used as a substrate for various glycosidases and glycosyltransferases.
• Used as a scaffold for the synthesis of various glycan epitopes such as sialyl Lewis X structures.

Key Features and Benefits:

• Excitation at 649 nm and emission at 671 nm.
• The fluorescent dye Cy5 is conjugated to the C6 position of the core-6 fucose.
• Can be separated on 15%-17% SDS-PAGE and directly visualized as a single band under a fluorescent imager.
• Linear response range for Cy5-labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.
• Store the unopened product at < -20 °C. Good for 6 months from date of receipt.

For Details:

• Wu et al., (2019) Glycobiology 29:750-754
• Wu et al., (2020) Glycobiology 30:970-980
• A Simple SDS-PAGE-based Technique for Probing Terminal Glycans

Related Reagents

Click Chemistry

• GDP-Cy5-Fucose (ES301)
• CMP-Cy5-Sialic Acid (ES302)
• GDP-Cy3-Fucose (ES401)
• CMP-Cy3-Sialic Acid (ES402)

Enzymes and detection reagents

• Various sialyltransferases
• Various neuraminidases/sialidases

Data Examples