Cy5-Fuc Labeled N3f (Cy5-N3f)

Applications

• Used as a ligand for lectins and to study glycan protein interaction.
• Used as a substrate for various glycosidase and glycosyltransferases.
• Used as a scaffold for the synthesis of various glycan epitopes.

Key Features:

• Excitation at 649 nm and emission at 671 nm.
• The fluorescent dye Cy5 is conjugated to the C6 position of the core-6 fucose.
• Can be separated on 15-17% SDS-PAGE and directly visualized as a single band through the red channel of a fluorescent imager.
• Linear response range for Cy5-labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.

Related Reagents

Labeled Glycans

• Cy5-Fuc Labeled M1N1f (GL301)
• Cy5-Fuc Labeled Glycan G2f (GL302)
• Cy5-Fuc Labeled N2f (GL304)
• Fluorescent Glycan Labeling and Detection

Click Chemistry

• GDP-Cy5-Fucose (ES301)
• CMP-Cy5-Sialic Acid (ES302)
• GDP-Cy3-Fucose (ES401)
• CMP-Cy3-Sialic Acid (ES402)

Enzymes and detection reagents

• Various sialytransferases
• Various fucosyltransferases
• Various neuraminidases/sialidases