Are R&D Systems Caspase Inhibitors irreversible?

Yes, the majority of R&D Systems Caspase Inhibitors have a Fluoromethyl ketone (FMK) functional group on the C-terminus of the peptide, and act as effective irreversible inhibitors with no added cytotoxic effects. Inhibitors synthesized with a benzyloxycarbonyl group (also known as BOC or Z) at the N-terminus and O-methyl side chains exhibit enhanced cellular permeability. R&D Systems also offers a General Caspase Inhibitor, Q-VD-OPh, Catalog # OPH001, as well as a FITC-conjugated pan-caspase inhibitor (ApoStat), Catalog # FMK012, which are both cell-permeable, irreversible inhibitors of caspase activity.

Does R&D Systems offer a negative control for Caspase Inihibitors with benzyloxycarbonyl group (Z-) at the N-terminus and the FMK functional group at the C-terminus?

Yes, R&D Systems offers Caspase Inhibitor Control Z-FA-FMK, Catalog # FMKC01, which is an inhibitor of cathepsins B and L but not caspases, and has been used in several systems as a negative control for peptide inhibitors of caspases.

Caspase-2 Inhibitor Z-VDVAD-FMK

Name: Caspase-2 Inhibitor Z-VDVAD-FMK
Brand: R&D Systems
SKU: FMK003

R&D Systems, part of Bio-Techne | Catalog # FMK003

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Product Datasheet / COA / SDS

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Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Cell permeable fluoromethyl ketone (FMK)-derivatized peptides act as effective irreversible Caspase inhibitors with no cytotoxic effects and, therefore, are useful tools for studying Caspase activity.

Conjugate

Unconjugated

View all Caspase-2 Small Molecules and Peptides »

Formulation, Preparation and Storage

FMK003

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Caspase-2

Caspase-2, also known as NEDD2 and ICH-1, is a 30â€‘32 kDa member of the peptidase C14A/ILâ€‘1 betaâ€‘converting family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-2 is a disulfide-linked homodimer, with activation occurring following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome. Initially, Pro-Caspase-2 is cleaved into a p34 subunit and a p14 subunit which are further processed to generate the prodomain, plus the mature p18 and p12 subunits. Notably, each p18-p12 heterodimer demonstrates proteolytic activity and localizes to either the nucleus or cytoplasm.

Alternate Names

CASP2, Caspase2, ICH-1, NEDD2

Entrez Gene IDs

835 (Human); 12366 (Mouse)

Gene Symbol

CASP2

Additional Caspase-2 Products

Product Documents for Caspase-2 Inhibitor Z-VDVAD-FMK

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COA

SDS

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Product Specific Notices for Caspase-2 Inhibitor Z-VDVAD-FMK

For research use only

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