Bumetanide

Name: Bumetanide
Brand: Tocris Bioscience
SKU: 3108

Catalog # 3108 | Tocris Bioscience a Bio-Techne Brand

Citations (12)

COA / SDS

Na+/2Cl-/K+ (NKCC) symporter inhibitor

Key Product Details

Description:	Na⁺/2Cl^-/K⁺ (NKCC) symporter inhibitor
Chemical Name:	3-(Aminosulfonyl)-5-(butylamino)-4-phenoxybenzoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	364.42

View Full Technical Details for Bumetanide

Technical Data
Citations (12) / Reviews

Biological Activity for Bumetanide

Bumetanide is a loop diuretic that inhibits the Na⁺/2Cl^-/K⁺ (NKCC) cotransporter. More potent than furosemide (Cat. No. 3109). Bumetanide improves electrophysiological, pathological and cognitive deficits in APOE4 knock-in mice. RNA sequencing of neurons derived from APOE4 induced pluripotent stem cell (iPSC) shows Bumetanide reverses Alzheimer's disease transcriptomic signatures.

Compound Libraries for Bumetanide

Bumetanide is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Bumetanide

M. Wt	364.42
Formula	C₁₇H₂₀N₂O₅S
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	28395-03-1
PubChem ID	2471
InChi Key	MAEIEVLCKWDQJH-UHFFFAOYSA-N
Smiles	CCCCNC1=CC(C(O)=O)=CC(S(=O)(N)=O)=C1OC2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Bumetanide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	36.44	100

Preparing Stock Solutions for Bumetanide

The following data is based on the product molecular weight 364.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.74 mL	13.72 mL	27.44 mL
5 mM	0.55 mL	2.74 mL	5.49 mL
10 mM	0.27 mL	1.37 mL	2.74 mL
50 mM	0.05 mL	0.27 mL	0.55 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Bumetanide

References are publications that support the biological activity of the product.

Taubes Experimental and real-world evidence supporting the computational repurposing of bumetanide for APOE4-related Alzheimer's disease. Nature Aging 2021 PMID: 36172600
Morita Functional evidence for involvement of bumetanide-sensitive Na⁺K⁺2Cl^- cotransport in the hepatoportal Na⁺ receptor of the sprague-dawley rat. Neurosci.Letts. 1999 PMID: 10320015
Isenring and Forbush Ion and bumet. binding by the Na-K-Cl cotransporter. J.Biol.Chem. 1997 PMID: 9305921
O'Grady Characteristics and functions of Na-K-Cl cotransport in epithelial tissues. Am.J.Physiol. 1987 PMID: 3303961

Citations for Bumetanide (12)

Showing 1 - 10 of 12 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Bumetanide include:

Babola et al. (2020) Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space. Elife. 9:e52160
PMID: 31913121
Spoljaric et al. (2019) KCC2-Mediated Cl- Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice. Cell Rep. 26:1073
PMID: 30699338
Bhandage et al. (2019) Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling. Front Cell Infect Microbiol. 9:73
PMID: 30949457
Alfonsa et al. (2016) Cl- out is a novel cooperative optogenetic tool for extruding chloride from neurons Nature Communications. 7:13495
PMID: 27853135
Choe et al. (2015) High salt intake increases blood pressure via BDNF-mediated downregulation of KCC2 and impaired baroreflex inhibition of vasopressin neurons. Mol Brain. 85:549
PMID: 25619659
Pál et al. (2015) Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity. Neuron. 8:35
PMID: 26043770
Angelantonio et al. (2014) A role for intracellular zinc in glioma alteration of neuronal chloride equilibrium. Cell Death Dis. 5:e1501
PMID: 25356870
Deeb et al. (2013) Disrupted Cl(-) homeostasis contributes to reductions in the inhibitory efficacy of D.pam during hyperexcited states. Eur J Neurosci. 38:2453
PMID: 23627375
Haam et al. (2012) GABA is excitatory in adult vasopressinergic neuroendocrine cells. J Neurosci. 32:572
PMID: 22238092
Shulga et al. (2012) The loop diuretic bumet. blocks posttraumatic p75NTR upregulation and rescues injured neurons. J Neurosci. 32:1757
PMID: 22302815
Shulga et al. (2008) Posttraumatic GABA(A)-mediated [Ca2+]i increase is essential for the induction of brain-derived neurotrophic factor-dependent survival of mature central neurons. J Neurosci. 28:6996
PMID: 18596173
Safiulina et al. (2008) Low expression of Kv7/M channels facilitates intrinsic and network bursting in the developing rat hippocampus. J Physiol. 586:5437
PMID: 18801845

There are no citations that match your criteria.