BromoTag^® AGB3

Product Description

BromoTag^® ABG3 is a selective "Bump & Hole" TAG Degrader (DC₅₀ 210 - 290 nM, protein degradation t½ = 113 min, D_max = 92%). BromoTag AGB3 comprises a VHL E3 ligase ligand joined by a linker to a (+)-JQ1 (Cat. No. 4499) derivative (the "bump" bearer) that selectively binds the Brd4^{BD2 L387A} variant (the "hole"). In cell lines expressing a fusion of the target protein with the tag, Brd4^{BD2 L387A}, BromoTag AGB3 drives formation of a ternary complex between VHL and the "BromoTagged" protein, leading to ubiquitination of the protein and its subsequent proteasomal degradation.

BromoTag^® is a registered trademark of the University of Dundee and is used under license.

Brd4^{BD2 L387A} can be expressed as a fusion with a target protein of interest using genome engineering techniques via CRISPR-mediated locus-specific knock-in. See protocol for more information.

Licensing Information

Sold under exclusive licence from the University of Dundee

Solubility

Preparing Stock Solutions for BromoTag^® AGB3

The following data is based on the product molecular weight 1030.70

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.97 mL	4.85 mL	9.70 mL
5 mM	0.19 mL	0.97 mL	1.94 mL
10 mM	0.10 mL	0.49 mL	0.97 mL
50 mM	0.02 mL	0.10 mL	0.19 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Bond Development of BromoTag: a 'Bump-and-Hole'-PROTAC system to induce potent, rapid, and selective degradation of tagged target proteins. J.Med.Chem. 2021 PMID: 34652918