Benzamil

Name: Benzamil
Brand: Tocris Bioscience
SKU: 3380

Tocris Bioscience a Bio-Techne Brand

Catalog # 3380

Citations (5)

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Key Product Details

Description:	NCX inhibitor, Deg/ENaC channel blocker; amiloride (Cat. No. 0890) derivative
Chemical Name:	N-(Benzylamidino)-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	356.21

View Full Technical Details for Benzamil

Biological Activity for Benzamil

Benzamil is a Na⁺/Ca²⁺ exhanger (NCX) inhibitor (IC₅₀ ~ 100 nM); TRPP3 channel blocker (IC₅₀ = 1.1 μM for inhibition of Ca²⁺-activated TRPP3 channel activity). Also non-selective Deg/ENaC family blocker; reduces mechanosensitivity of colonic afferents. More potent derivative of amiloride (Cat. No. 0890).

Compound Libraries for Benzamil

Benzamil is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Benzamil

M. Wt	356.21
Formula	C₁₃H₁₄ClN₇O.HCl
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	161804-20-2
PubChem ID	5702295
InChi Key	ZNWMRWWNJBXNKJ-UHFFFAOYSA-N
Smiles	NC1=C(Cl)N=C(C(NC(NCC2=CC=CC=C2)=N)=O)C(N)=N1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Benzamil

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	35.62	100
	water	3.56	10

Preparing Stock Solutions for Benzamil

The following data is based on the product molecular weight 356.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.81 mL	14.04 mL	28.07 mL
5 mM	0.56 mL	2.81 mL	5.61 mL
10 mM	0.28 mL	1.40 mL	2.81 mL
50 mM	0.06 mL	0.28 mL	0.56 mL

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References for Benzamil

References are publications that support the biological activity of the product.

Dai Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 2007 PMID: 17804601
Page Acid sensing ion channels 2 and 3 are required for inhibition of visceral nociceptors by benzamil. Pain 2007 PMID: 17467171
Fischer Characterization of a Na⁺-Ca²⁺ exchanger in podocytes. Nephrol.Dial.Transplant. 2002 PMID: 12270979

Citations for Benzamil (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Benzamil include:

Mizuno et al. (2019) Development of Orthogonal Linear Separation Analysis (OLSA) to Decompose Drug Effects into Basic Components. Sci Rep. 9:1824
PMID: 30755704
Panja et al. (2019) Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity. Neuropathol Appl Neurobiol. 45:141
PMID: 29679372
Singh (2018) Determination of system level alterations in host transcriptome due to Zika virus (ZIKV) Infection in retinal pigment epithelium. Sci Rep. 8:11209
PMID: 30046058
Zou et al. (2018) Knockout of mitochondrial voltage-dependent anion channel type 3 increases reactive oxygen species (ROS) levels and alters renal sodium transport. J Biol Chem. 293:1666
PMID: 29180450
Agarwal et al. (2017) Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes. Neuron. 93:587
PMID: 28132831

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Showing 1 - 5 of 5 citations Showing All

Product Documents for Benzamil

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.