Benzamil

Name: Benzamil
Brand: Tocris Bioscience
SKU: 3380

Tocris Bioscience, part of Bio-Techne | Catalog # 3380

Citations (11)

COA / SDS

NCX inhibitor, Deg/ENaC channel blocker; amiloride (Cat. No. 0890) derivative

Key Product Details

Description:	NCX inhibitor, Deg/ENaC channel blocker; amiloride (Cat. No. 0890) derivative
Chemical Name:	N-(Benzylamidino)-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	356.21

View Full Technical Details for Benzamil

Technical Data
Citations

Biological Activity for Benzamil

Benzamil is a Na⁺/Ca²⁺ exhanger (NCX) inhibitor (IC₅₀ ~ 100 nM); TRPP3 channel blocker (IC₅₀ = 1.1 μM for inhibition of Ca²⁺-activated TRPP3 channel activity). Also non-selective Deg/ENaC family blocker; reduces mechanosensitivity of colonic afferents. More potent derivative of amiloride (Cat. No. 0890).

Compound Libraries for Benzamil

Benzamil is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Benzamil

M. Wt	356.21
Formula	C₁₃H₁₄ClN₇O.HCl
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	161804-20-2
PubChem ID	5702295
InChi Key	ZNWMRWWNJBXNKJ-UHFFFAOYSA-N
Smiles	NC1=C(Cl)N=C(C(NC(NCC2=CC=CC=C2)=N)=O)C(N)=N1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Benzamil

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	35.62	100
	water	3.56	10

Preparing Stock Solutions for Benzamil

The following data is based on the product molecular weight 356.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.81 mL	14.04 mL	28.07 mL
5 mM	0.56 mL	2.81 mL	5.61 mL
10 mM	0.28 mL	1.40 mL	2.81 mL
50 mM	0.06 mL	0.28 mL	0.56 mL

Molarity Calculator

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Reconstitution Calculator

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References for Benzamil

References are publications that support the biological activity of the product.

Fischer Characterization of a Na⁺-Ca²⁺ exchanger in podocytes. Nephrol.Dial.Transplant. 2002 PMID: 12270979
Page Acid sensing ion channels 2 and 3 are required for inhibition of visceral nociceptors by benzamil. Pain 2007 PMID: 17467171
Dai Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 2007 PMID: 17804601