5-Azacytidine

Name: 5-Azacytidine
Brand: Tocris Bioscience
SKU: 3842

Tocris Bioscience a Bio-Techne Brand

Catalog # 3842

Citations (5)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	DNA methyltransferase inhibitor
Chemical Name:	4-Amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one
Purity:	≥98% (HPLC)
Molecular Weight:	244.2

View Full Technical Details for 5-Azacytidine

Biological Activity for 5-Azacytidine

5-Azacytidine is a DNA methyltransferase inhibitor. Incorporates into DNA forming covalent adducts with cellular DNMT1, depleting enzyme activity. Induces demethylation and reactivation of silenced genes. Improves the efficiency of reprogramming of stem cells; induces differentiation of mesenchymal stem cells into cardiomyocytes.

Compound Libraries for 5-Azacytidine

5-Azacytidine is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for 5-Azacytidine

M. Wt	244.2
Formula	C₈H₁₂N₄O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	320-67-2
PubChem ID	9444
InChi Key	NMUSYJAQQFHJEW-KVTDHHQDSA-N
Smiles	O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N2C(N=C(N)N=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5-Azacytidine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	12.21	50
	DMSO	24.42	100

Preparing Stock Solutions for 5-Azacytidine

The following data is based on the product molecular weight 244.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.10 mL	20.48 mL	40.95 mL
5 mM	0.82 mL	4.10 mL	8.19 mL
10 mM	0.41 mL	2.05 mL	4.10 mL
50 mM	0.08 mL	0.41 mL	0.82 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for 5-Azacytidine

References are publications that support the biological activity of the product.

Qian 5-Azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated kinase. Stem Cells Dev. 2012 PMID: 21476855
Mikkelsen Dissecting direct reprogramming through integrative genomic analysis. Nature 2008 PMID: 18509334
Schneider-Stock 5-aza-Cytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms. J.Pharmacol.Exp.Ther. 2005 PMID: 15547111

Citations for 5-Azacytidine (5)

Showing 1 - 5 of 5 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for 5-Azacytidine include:

Linscott and Chung (2019) TET1 regulates fibroblast growth factor 8 transcription in Gn releasing hormone neurons. PLoS One. 14:e0220530
PMID: 31361780
Chandrakanthan et al. (2016) PDGF-AB and 5-Azacytidine induce conversion of somatic cells into tissue-regenerative multipotent stem cells Proc Natl Acad Sci U S A. 113:E2306
PMID: 27044077
Bhuvanagiri et al. (2014) 5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion. EMBO Mol Med. 6:1593
PMID: 25319547
Chong et al. (2013) Progenitor cells identified by PDGFR-α expression in the developing and diseased human heart. Cell Res. 22:1932
PMID: 23391309
Perera et al. (2009) Relation of DNA methylation of 5'-CpG island of ACSL3 to transplacental exposure to airborne polycyclic aromatic hydrocarbons and childhood asthma. PLoS One. 4:e4488
PMID: 19221603

There are no citations that match your criteria.

Showing 1 - 5 of 5 citations Showing All

Product Documents for 5-Azacytidine

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Azacitidine, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov