5-Azacytidine
Tocris Bioscience, part of Bio-Techne | Catalog # 3842
DNA methyltransferase inhibitor
Key Product Details
Description
DNA methyltransferase inhibitor
Product Description
5-Azacytidine is a DNA methyltransferase inhibitor. Incorporates into DNA forming covalent adducts with cellular DNMT1, depleting enzyme activity. Induces demethylation and reactivation of silenced genes. Improves the efficiency of reprogramming of stem cells; induces differentiation of mesenchymal stem cells into cardiomyocytes.Product Specifications
Molecular Weight
244.20
Formula
C8H12N4O5
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
4-Amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one
CAS Number
320-67-2
PubChem ID
9444
InChI Key
NMUSYJAQQFHJEW-KVTDHHQDSA-N
SMILES
O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N2C(N=C(N)N=C2)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 12.21 | 50 | |
| DMSO | 24.42 | 100 |
Preparing Stock Solutions
for 5-Azacytidine
The following data is based on the product molecular weight 244.20
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.10 mL | 20.48 mL | 40.95 mL |
| 5 mM | 0.82 mL | 4.10 mL | 8.19 mL |
| 10 mM | 0.41 mL | 2.05 mL | 4.10 mL |
| 50 mM | 0.08 mL | 0.41 mL | 0.82 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Mikkelsen Dissecting direct reprogramming through integrative genomic analysis. Nature 2008 PMID: 18509334
- Qian 5-Azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated kinase. Stem Cells Dev. 2012 PMID: 21476855
- Schneider-Stock 5-aza-Cytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms. J.Pharmacol.Exp.Ther. 2005 PMID: 15547111
Product Specific Notices for 5-Azacytidine
For research use only
⚠ WARNING: This product can expose you to chemicals including Azacitidine, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov
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