4-Aminopyridine is a non-selective voltage-dependent K⁺-channel blocker (IC₅₀ values are 170 and 230 μM at K_V1.1 and K_V1.2 respectively). Caged 4-AP (Cat. No. 3557) is also available.

Product Specifications

Molecular Weight

94.12

Formula

C₅H₆N₂

Storage

Store at RT

Purity

≥98% (HPLC)

CAS Number

504-24-5

PubChem ID

1727

InChI Key

NUKYPUAOHBNCPY-UHFFFAOYSA-N

SMILES

NC1=CC=NC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	9.41	100
	DMSO	9.41	100

Preparing Stock Solutions for 4-Aminopyridine

The following data is based on the product molecular weight 94.12

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	10.62 mL	53.12 mL	106.25 mL
5 mM	2.12 mL	10.62 mL	21.25 mL
10 mM	1.06 mL	5.31 mL	10.62 mL
50 mM	0.21 mL	1.06 mL	2.12 mL

Calculators

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

Background References

References are publications that support the biological activity of the product.

Tseng Different state dependencies of 4-aminopyridine binding to rKv1.4 and rKv4.2: role of the cytoplasmic halves of the fifth and sixth transmembrane segments. J.Pharmacol.Exp.Ther. 1999 PMID: 10411564
Smith In vitro electrophysiological activity of nerispirdine, a novel 4-aminopyridine derivative. Clin.Exp.Pharmacol.Physiol. 2009 PMID: 19413590
Tseng Reverse use dependence of Kv4.2 blockade by 4-aminopyridine. J.Pharmacol.Exp.Ther. 1996 PMID: 8930194
Bouchard and Fedida Closed and open state binding of 4-aminopyridine to the cloned human potassium channel Kv1.5. J.Pharmacol.Exp.Ther. 1995 PMID: 7473178

4-Aminopyridine

Tocris Bioscience, part of Bio-Techne | Catalog # 0940

Key Product Details

Description

Alternative Names

View all Voltage-gated Potassium Channel Blockers »

Product Description