Simple Plex Mouse IL-18 Cartridge

Name: Simple Plex Mouse IL-18 Cartridge
Brand: ProteinSimple
SKU: SPCKC-MP-012862

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-MP-012862

Product Datasheet / COA

Simple Plex Mouse IL-18 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD21, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	6.05 pg/mL
Assay Range:	10.1 - 38,400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse IL-18 Cartridge Summary:	Simple Plex assay is for the detection of mouse IL-18 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD21.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse IL-18 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse IL-18 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	81	79
Mean (pg/mL)	489	19203	337	15167
Standard Deviation	20	754	50	1992
CV%	4.1	3.9	14.7	13.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	99	99-100
EDTA Plasma (n=4)	90	78-104
Heparin Plasma (n=4)	88	83-93
Serum (n=4)	84	77-90

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse IL-18 Cartridge

	Simple Plex Mouse IL-18 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.

Item	Value
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse IL-18 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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