Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-003377
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-1 beta/IL-1F2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-1 beta/IL-1F2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-1 beta/IL-1F2.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 135 | 144 |
| Mean (pg/mL) | 54.8 | 2711 | 51.1 | 2529 |
| Standard Deviation | 1.87 | 75.3 | 6.22 | 228 |
| CV% | 3.4 | 2.8 | 12.2 | 9.0 |
Recovery for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
Recovery for the mouse IL-1 beta/IL-1F2 assays was evaluated at three different spiked concentrations of IL-1 beta/IL-1F2 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 105 | 88-118 |
| Heparin Plasma (n=4) | 105 | 100-112 |
| Serum (n=4) | 89 | 83-97 |
Linearity
Samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
Simple Plex Mouse IL-1 beta/IL-1F2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-1 beta/IL-1F2 Assay. Assay range is 0.88-3360 pg/mL.
Simple Plex Mouse IL-1 beta/IL-1F2 Assay Endogenous Levels
Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-1 beta/IL-1F2 Products
Product Documents for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
Product Specific Notices for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
For research use only