Simple Plex Human IL-18 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000501
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-18 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-18 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-18 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-18.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 15 | 14 |
| Mean (pg/mL) | 10.5 | 569 | 10.3 | 587 |
| Standard Deviation | 0.524 | 18.7 | 0.882 | 58.4 |
| CV% | 5.2 | 3.3 | 8.6 | 10.0 |
Recovery for Simple Plex Human IL-18 Cartridge
Recovery for the human IL-18 assays was evaluated at three different spiked concentrations of IL-18 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Urine (n=5) | 120 | 114-128 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-18 Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-18 Assay. Assay range is 2.40-3660 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: IL-18/IL-1F4
IL-18 (Interleukin-18) is secreted by macrophages, dendritic cells, and epithelial cells. Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity. IL-18 signals through a receptor complex that contains IL-18 R alpha IL-18 R beta. It synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma. It can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses. Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome. In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion.
Long Name
Alternate Names
Gene Symbol
Additional IL-18/IL-1F4 Products
Product Documents for Simple Plex Human IL-18 Cartridge
Product Specific Notices for Simple Plex Human IL-18 Cartridge
For research use only