Simple Plex Human ICAM-1/CD54 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000303
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human ICAM-1/CD54 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 117 | 5027 | 106 | 5096 |
| Standard Deviation | 6.60 | 318 | 6.78 | 245 |
| CV% | 5.7 | 6.3 | 6.4 | 4.8 |
Recovery for Simple Plex Human ICAM-1/CD54 Cartridge
Recovery was not necessary as ICAM-1 shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of human ICAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human ICAM-1/CD54 Cartridge
Human ICAM-1 Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: ICAM-1/CD54
ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
Long Name
Alternate Names
Gene Symbol
Additional ICAM-1/CD54 Products
Product Documents for Simple Plex Human ICAM-1/CD54 Cartridge
Product Specific Notices for Simple Plex Human ICAM-1/CD54 Cartridge
For research use only