Simple Plex Human HVEM/TNFRSF14 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003550
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human HVEM/TNFRSF14 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one HVEM assay.
Inter-Assay Precision (Precision between assays) Replicates of each HVEM control were tested in multiple assays performed by at least three technicians using two lots of reagents for human HVEM.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 34 | 34 |
| Mean (pg/mL) | 96.8 | 4590 | 95.2 | 4678 |
| Standard Deviation | 8.14 | 348 | 9.93 | 365 |
| CV% | 8.4 | 7.6 | 10.4 | 7.8 |
Recovery for Simple Plex Human HVEM/TNFRSF14 Cartridge
Recovery was not necessary as human HVEM shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human HVEM were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human HVEM/TNFRSF14 Cartridge
Simple Plex Human HVEM Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human HVEM Assay. Assay range is 1.70-16,240 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: HVEM/TNFRSF14
HVEM/TNFRSF14 (herpesvirus entry mediator) is a transmembrane protein that is most highly expressed on naïve, memory, and regulatory T cells but declines during T cell activation. It is present at low levels on most resting leukocytes. HVEM is a receptor for BTLA, CD160, and LIGHT. A cis interaction between HVEM and BTLA promotes cell survival, while trans interaction promotes a state of lymphocyte inactivity. LIGHT is expressed following T cell activation and competes with BTLA for HVEM binding, leading to a proinflammatory response. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding. Human, but not mouse, HVEM can also bind lymphotoxin-alpha.
Long Name
Alternate Names
Gene Symbol
Additional HVEM/TNFRSF14 Products
Product Documents for Simple Plex Human HVEM/TNFRSF14 Cartridge
Product Specific Notices for Simple Plex Human HVEM/TNFRSF14 Cartridge
For research use only