Simple Plex Human gp130 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000494
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human gp130 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one gp130 assay.
Inter-Assay Precision (Precision between assays) Replicates of each gp130 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human gp130.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 52 | 49 |
| Mean (pg/mL) | 169 | 7365 | 181 | 8257 |
| Standard Deviation | 4.97 | 222 | 17.7 | 737 |
| CV% | 2.9 | 3.0 | 9.8 | 8.9 |
Recovery for Simple Plex Human gp130 Cartridge
Recovery for the human gp130 assays was evaluated at three different spiked concentrations of gp130 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=2) | 107 | 107-108 |
Linearity
Samples containing and/or spiked with high concentrations of human gp130 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human gp130 Cartridge
Simple Plex Human gp130 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human gp130 Assay. Assay range is 6.19-23,640 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R.
Long Name
Alternate Names
Gene Symbol
Additional gp130 Products
Product Documents for Simple Plex Human gp130 Cartridge
Product Specific Notices for Simple Plex Human gp130 Cartridge
For research use only