Non-Human Primate CTLA-4 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011349
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Non-Human Primate CTLA-4 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 54 | 51 |
| Mean (pg/mL) | 209 | 9821 | 212 | 9517 |
| Standard Deviation | 8.97 | 616 | 16.4 | 701 |
| CV% | 4.3 | 6.3 | 7.7 | 7.4 |
Recovery for Non-Human Primate CTLA-4 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 103 | 100-105 |
| Heparin Plasma (n=4) | 104 | 101-109 |
| Serum (n=5) | 105 | 88-114 |
Linearity
Samples containing and/or spiked with high concentrations of CTLA-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Non-Human Primate CTLA-4 Cartridge
Non-Human Primate CTLA-4 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CTLA-4
CTLA-4/CD152 (Cytotoxic T-Lymphocyte Antigen 4) is a transmembrane T cell inhibitory molecule that is expressed as a covalent homodimer. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending CD28-dependent T cell activation. Mice deleted for CTLA-4 develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells which constitutively express CTLA-4.
Long Name
Alternate Names
Gene Symbol
Additional CTLA-4 Products
Product Documents for Non-Human Primate CTLA-4 Cartridge
Product Specific Notices for Non-Human Primate CTLA-4 Cartridge
For research use only