Horse anti-Mouse M.O.M.(R) (Mouse-On-Mouse) IgG Secondary Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # MKB-2225-NB

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MKB-2225-NB
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Key Product Details

Species Reactivity

Mouse

Applications

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Polyclonal Horse IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all M.O.M.(R) (Mouse-On-Mouse) IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Clonality

Polyclonal

Host

Horse

Isotype

IgG

Description

This unit of Horse anti-Mouse, M.O.M.(R) (Mouse-On-Mouse) IgG Secondary Antibody [Biotin] has been optimized for use with the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit components. This is the same reagent contained in the following Vector(R) M.O.M.(R) Kits and is meant as a replacement unit:

PK-2200-NB

FMK-2201-NB

BMK-2202-NB

Applications

Application
Recommended Usage

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This reagent is supplied as 0.1 mL stock solution sufficient to make 25 mL of working solution..

Formulation, Preparation, and Storage

Purification

Affinity purified

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: M.O.M.(R) (Mouse-On-Mouse) IgG

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit. The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector(R) M.O.M.(R) (Mouse-On-Mouse) System. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

Additional M.O.M.(R) (Mouse-On-Mouse) IgG Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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