Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # eB121-15F9

Concentration

0.5 mg/ml

View all IgM Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgM

Specificity

This secondary antibody eacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugate can be used as a detection secondary for mouse IgM.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9)

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [NBP1-43282]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [NBP1-43282]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [NBP1-43282] - Flow Cytometry: Mouse IgM Antibody (eB121-15F9) [PE] [NBP1-43749] - See notes for image caption
Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [NBP1-43282]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [NBP1-43282]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [NBP1-43282] - Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 ug of Rat IgG2a Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE. Total viable cells were used for analysis.

Applications for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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