Key Product Details

Species Reactivity

Human

Applications

Dot Blot, ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Rabbit anti-Human IgG Fc fragment Secondary Antibody was produced by repeated immunization with Human IgG F(c) fragment in rabbit.

Specificity

No reaction was observed against Human IgG F(ab).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Human IgG Fc Secondary Antibody

Dot Blot: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - Dot Blot results of [FITC]. Dots are Human IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the FITC version of this antibody.
ELISA: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675]

ELISA: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675]

ELISA: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - ELISA results tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 ug of Human IgG F(c). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.
Rabbit anti-Human IgG Fc Secondary Antibody

Rabbit anti-Human IgG Fc Secondary Antibody

Western Blot of Rabbit anti-Human Fc antibody. Lane 1: Human Fc

Applications for Rabbit anti-Human IgG Fc Secondary Antibody

Application
Recommended Usage

ELISA

1:375000

Immunohistochemistry

1:1000-1:6000

Western Blot

1:3000-1:30000
Application Notes
This product has been tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Human IgG Fc Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Human IgG Fc Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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