Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72623
Key Product Details
Species Reactivity
Human
Applications
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rabbit anti-Human IgG Fc fragment Secondary Antibody was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
Specificity
No reaction was observed against Human IgG F(ab).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum
Scientific Data Images for Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]
Dot Blot: Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72623] - Antigen: Human IgG Fc Load: Lane 1 - 100 ng Lane 2 - 33.33 ng Lane 3 - 11.11 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng Incubated for 1 HR at RT; blocked for 1 HR at RT. Visualized using an alkaline phosphatase substrate.
Applications for Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]
Application
Recommended Usage
ELISA
1:2000 - 1:10000
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:2500
Application Notes
This product has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]
Product Specific Notices for Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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