Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody [DyLight 650] (Pre-adsorbed)

Mouse

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Polyclonal Goat IgG

Pre-adsorbed

1 mg/ml

Mouse IgG-F(ab')2 Fragment

Mouse. Minimum reactivity to human and rat.

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 650. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Polyclonal

Goat

IgG

DyLight 650 is excited at 652 (in PBS) and emits at 672 (in PBS).

DyLight 650 replaces DyLight 649.

Immunogen affinity purified

Phosphate Buffered Saline (PBS) containing 0.2% BSA.

Pre-adsorbed

0.09% Sodium Azide

1 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C. Do not freeze.