Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody [Biotin] (Pre-adsorbed)

Mouse

ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot

Biotin

Polyclonal Goat IgG

Pre-adsorbed

1 mg/ml

Mouse IgG-F(ab')2 Fragment

Mouse. Minimum reactivity to human and rat.

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to biotin. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Polyclonal

Goat

IgG

Immunogen affinity purified

Phosphate Buffered Saline (PBS) containing 0.2% BSA.

Pre-adsorbed

0.09% Sodium Azide

1 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C. Do not freeze.