Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73030
Conjugate
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Format
Pre-adsorbed
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Sheep IgG F(ab')2 fragment
Specificity
No reaction was observed against Sheep IgG F(c) or Human Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum
Applications for Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:100000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:5000
Application Notes
This product is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 500 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Format
Pre-adsorbed
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Specific Notices for Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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