Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72808
Conjugate
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Sheep IgG F(ab')2 fragment
Specificity
This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Sheep IgG F(c). This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but antibodies may cross-react with immunoglobulins from other species.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Applications for Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Format
Pre-adsorbed
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Documents for Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody (Pre-adsorbed)
Product Specific Notices for Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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