Rabbit anti-Human IgA Fc Secondary Antibody [FITC]

Human

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

FITC (Excitation = 495 nm, Emission = 519 nm)

Polyclonal Rabbit

LYOPH mg/ml

Highly purified normal IgA isolated from pooled human serum and a purified IgA2- . Freund's complete adjuvant is used in the first step of the immunization procedure.

The antiserum does not cross-react with any other component of the Human immunoglobulin system as tested in gel-immunodiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against human serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react with IgG, IgG/Fab fragments and IgM or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.

Polyclonal

Rabbit

The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Ion exchange chromatography

Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Lyophilized from PBS (pH 7.2)

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.