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Donkey Anti-Mouse IgG Biotinylated Antibody

Bio-Techne includes R&D Systems | Catalog # BAF018

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Donkey IgG

Product Summary for Donkey Anti-Mouse IgG Biotinylated Antibody

Immunogen

Mouse IgG

Specificity

Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISA and Western blots. In direct ELISA, less than 5% cross‑reactivity with human IgG, rabbit IgG, and chicken IgY is observed.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Applications for Donkey Anti-Mouse IgG Biotinylated Antibody

Application
Recommended Usage

Western Blot

Use at a 1:2000 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using BAF018 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Donkey Anti-Mouse IgG Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Donkey Anti-Mouse IgG Biotinylated Antibody

For research use only

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Donkey Anti-Mouse IgG Biotinylated Antibody

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

Showing  1 - 1 of 1 FAQ Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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