Recombinant Human VPS28 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30248

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NBP1-30248
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all VPS28 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-221 of Human VPS28

Source: E.coli

Amino Acid Sequence: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human VPS28 Protein

Western Blot: Recombinant Human VPS28 Protein [NBP1-30248]

Western Blot: Recombinant Human VPS28 Protein [NBP1-30248]

Western Blot: VPS28 Protein [NBP1-30248] - 15% SDS-PAGE (3ug)
SDS-PAGE: Recombinant Human VPS28 Protein [NBP1-30248]

SDS-PAGE: Recombinant Human VPS28 Protein [NBP1-30248]

SDS-Page: VPS28 Protein [NBP1-30248] - VPS28, 25.4 kDa (221aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-30248
Formulation 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS28

VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

ESCRT-I complex subunit VPS28, H-Vps28, MGC60323, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 28 homolog, yeast class E protein Vps28p homolog

Gene Symbol

VPS28

Additional VPS28 Products

Product Documents for Recombinant Human VPS28 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VPS28 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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