Talin2 Recombinant Protein Antigen
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62621PEP
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Antibody Competition
Product Specifications
Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human TLN2.
Source: E. coli
Amino Acid Sequence: AADLNQSAGEVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQVIGNLKNISMA
Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)
This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Purity
>80% by SDS-PAGE and Coomassie blue staining
Predicted Molecular Mass
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Antibody Competition (10-100 molar excess)
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-62621.
It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.
For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
Protein / Peptide Type
Recombinant Protein Antigen
Formulation, Preparation and Storage
NBP2-62621PEP
| Formulation | PBS and 1M Urea, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: Talin2
Talin2 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 240 kDa cytoplasmic focal adhesion molecule. It likely forms dimers that link integrins to the actin cytoskeleton. Human Talin2 is 2542 amino acids (aa) in length. It contains one globular N-terminal FERM domain that binds integrin cytodomains (aa 88-406), followed by an Ala-rich region (aa 861-1193) and a C-terminal actin-binding domain (aa 2321-2530). Human Talin2 has three potential variants. Two show alternate start sites at Met19 and Met1153, while a third has an alternate start site at Met1608 accompanied by a deletion of aa 1748-1790. Over aa's 977-1183, human Talin2 shares 99% and 97% aa identity with canine and mouse Talin2, respectively.
Alternate Names
ILWEQ, TLN2
Gene Symbol
TLN2
Additional Talin2 Products
Product Documents for Talin2 Recombinant Protein Antigen
Product Specific Notices for Talin2 Recombinant Protein Antigen
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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