Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77488

Novus Biologicals, part of Bio-Techne
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NBP2-77488-0.1mg
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Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Sulfatase Modifying Factor 1/SUMF1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a His-Tag and corresponding to the amino acids 34-374aa of Human Sulfatase Modifying Factor 1/SUMF1.

Source: Baculovirus

Amino Acid Sequence: SQEAGTGAGA GSLAGSCGCG TPQRPGAHGS SAAAHRYSRE ANAPGPVPGE RQLAHSKMVP IPAGVFTMGT DDPQIKQDGE APARRVTIDA FYMDAYEVSN TEFEKFVNST GYLTEAEKFG DSFVFEGMLS EQVKTNIQQA VAAAPWWLPV KGANWRHPEG PDSTILHRPD HPVLHVSWND AVAYCTWAGK RLPTEAEWEY SCRGGLHNRL FPWGNKLQPK GQHYANIWQG EFPVTNTGED GFQGTAPVDA FPPNGYGLYN IVGNAWEWTS DWWTVHHSVE ETLNPKGPPS GKDRVKKGGS YMCHRSYCYR YRCAARSQNT PDSSASNLGF RCAADRLPTM DHHHHHH

Purity

>85%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

38.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein

SDS-PAGE: Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein [NBP2-77488]

SDS-PAGE: Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein [NBP2-77488]

SDS-Page: Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein [NBP2-77488] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-77488
Formulation PBS (pH 7.4), 10% glycerol.
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sulfatase Modifying Factor 1/SUMF1

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD; MIM 272200), a lysosomal storage disorder (Roeser et al., 2006 [PubMed 16368756]).[supplied by OMIM]

Alternate Names

FGE1

Gene Symbol

SUMF1

Additional Sulfatase Modifying Factor 1/SUMF1 Products

Product Documents for Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Sulfatase Modifying Factor 1/SUMF1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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