Mouse Sonic Hedgehog/Shh Recombinant Protein

Name: Mouse Sonic Hedgehog/Shh Recombinant Protein
Brand: Novus Biologicals
SKU: NBP2-35339

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35339

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Sonic Hedgehog/Shh Proteins and Enzymes »

Product Specifications

Description

A biologically active protein to SHH.Source: E. coli

Purity

SDS-PAGE

Endotoxin Level

Less than 1 EU/mg of the rMuSHH as determined by LAL method.

Predicted Molecular Mass

19.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

The ED50, as determined by inducing alkaline phosphatase production using murine C3H10T1/2 cells, is less than 300 ng/ml.

Application Notes

Novus Biologicals' NovActive proteins are fully active and stringently purified to provide only the safest and highest efficacy proteins available. NovActive proteins are expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance. NovActive proteins are guaranteed to be greater than 90% pure by HPLC, and contain less than 0.005 EU/ug of endotoxin.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35339

Preparation Method	Lyophilized from 0.2 mm filtered concentrated solution in 5 mM PBS, pH 5.5, 150 mM NaCl, 0.5 mM DTT.
Concentration	Lyoph
Reconstitution	Centrifuge prior to opening. Reconstitute with sterilized PBS or aqueous buffer to a final concentration of 0.1 mg/ml.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a secreted protein that regulates developmental morphogenesis of the central nervous system, somite, limb, whisker and hair follicles, foregut, tooth, and bone. Shh is also involved in regulating neural and hematopoeitic stem cell fates and determination of the T cell repertoire. Aberrant Shh signaling is implicated in the development of several malignancies. Shh undergoes autocatalytic processing that yields a palmitoylated N-terminal fragment and a C-terminal fragment. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain acts as a cholesterol transferase. Shh activity is mediated by a multicomponent receptor complex that includes Patched and Smoothened. The N-terminal fragment of Shh is greater than 98% conserved between mouse, human, rat, canine, porcine, and chicken.

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Gene Symbol

SHH

Additional Sonic Hedgehog/Shh Products

Product Documents for Mouse Sonic Hedgehog/Shh Recombinant Protein

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COA

Product Specific Notices for Mouse Sonic Hedgehog/Shh Recombinant Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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